LOCUS       sequence-154535-         693 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically 
            selected. Modified from BBa_J23100. [F:36INS-J23100:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 86 to 120)
  AUTHORS   Carr SB, Beal J, Densmore DM
  TITLE     Reducing DNA context dependence in bacterial promoters.
  JOURNAL   PLoS One. 2017 Apr 19;12(4):e0176013. doi: 
            10.1371/journal.pone.0176013. eCollection 2017.
  PUBMED    28422998
REFERENCE   2  (bases 1 to 693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-04-19"; volume: "12(4):e0176013. doi"; pages: " 
            10.1371/journal.pone.0176013. eCollection 2017"
COMMENT     Sequence Label: 36INS-J23100_FB
FEATURES             Location/Qualifiers
     source          1..693
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..48
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    54..74
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     promoter        complement(86..120)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    175..196
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      199..242
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(246..264)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        332..436
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(655..674)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcctgcagc
       61 ggccgctact agtaggtctc tagtagctag cactgtacct aggactgagc tagccgtcaa
      121 atctaccttc cgtaagctga aaatgaatac tgcaggagcg tgagacccac tagtctctag
      181 aagcggccgc gaattccaga aatcatcctt agcgaaagct aaggattttt tttatctgaa
      241 attctgcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
      301 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      361 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      421 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      481 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      541 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      601 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      661 tggcgcggta ttatcccgta ttgacgccgg gca
//
