LOCUS       sequence-154539-         524 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  36INS-J23100_GB Insulated MoClo promoter for E. coli. 36nt DNA 
            spacer empirically selected. Modified from BBa_J23100. 
            [G:36INS-J23100:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 69 to 103)
  AUTHORS   Carr SB, Beal J, Densmore DM
  TITLE     Reducing DNA context dependence in bacterial promoters.
  JOURNAL   PLoS One. 2017 Apr 19;12(4):e0176013. doi: 
            10.1371/journal.pone.0176013. eCollection 2017.
  PUBMED    28422998
REFERENCE   2  (bases 1 to 524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-04-19"; volume: "12(4):e0176013. doi"; pages: " 
            10.1371/journal.pone.0176013. eCollection 2017"
COMMENT     Sequence Label: 36INS-J23100_GB
FEATURES             Location/Qualifiers
     source          1..524
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     14..31
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    37..57
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     promoter        complement(69..103)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_feature    158..179
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      182..225
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(229..247)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        315..419
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aacgaccgag cgcagcgagt cagtgagcga ggaagcctgc agcggccgct actagtaggt
       61 ctctagnagc tagcactgta cctaggactg agctagccgt caaatctacc ttccgtaagc
      121 tgaaaatgaa tactgcaggg gcatgagacc cactagtctc tagaagcggc cgcgaattcc
      181 agaaatcatc cttagcgaaa gctaaggatt ttttttatct gaaattctgc ctcgtgatac
      241 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      301 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      361 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      421 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      481 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaa
//
