LOCUS       sequence-154543-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically 
            selected.Modified from BBa_I13453 to adjust spacing in MoClo system.
            [A:36INS1-pBAD:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Carr SB, Beal J, Densmore DM
  TITLE     Reducing DNA context dependence in bacterial promoters.
  JOURNAL   PLoS One. 2017 Apr 19;12(4):e0176013. doi: 
            10.1371/journal.pone.0176013. eCollection 2017.
  PUBMED    28422998
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-04-19"; volume: "12(4):e0176013. doi"; pages: " 
            10.1371/journal.pone.0176013. eCollection 2017"
COMMENT     Sequence Label: 36INS1-pBAD_AB
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..20
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(24..67)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    70..91
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     179..198
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    287..307
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(313..330)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(564..583)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaataggcgt atcacgaggc agaatttcag ataaaaaaaa tccttagctt tcgctaagga
       61 tgatttctgg aattcgcggc cgcttctaga gactagtggg tctcaggagc gttgttcgcg
      121 accctgttct gaggcaatgt tgggcacatt gattatttgc acggcgtcac actttgctat
      181 gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc
      241 tctactgttt ctccataccg tttttttggg ctagctacta gagacctact agtagcggcc
      301 gctgcaggct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      361 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      421 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      481 gtttttccac aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      541 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      601 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      661 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      721 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      781 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
      841 tggtaacagg attagcagag cgag
//
