LOCUS       sequence-154552-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-ER-3
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             92..769
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     493..512
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     687..710
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gctggtttag tgaaccgtca gatccgctag catgctgcta tccgtgccgt tgctgctcgg
       61 cctcctcggc ctggccgtcg ccgaccggtc gatggtgagc aagggcgagg aggtcatcaa
      121 ggagttcatg cgcttcaagg agcacatgga gggctccgtg aacggccacg agttcgagat
      181 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaggc tgaaggtgac
      241 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagatca tgtacggctc
      301 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
      361 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
      421 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aaggtgcgcg gcaccaactt
      481 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
      541 gatgtacccc gaggacggcg ccctgaaggg cgagatgaag atgaggctga ggctgaagga
      601 cggcggccac tacgacgccg aggtcaagac cacctacatg gccaagaagc ccgtgcagct
      661 gcccggcgcc tacaagaccg acatcaagct ggacatcacc tcccacaacg aggactacac
      721 catcgtggaa cagtacgagc gcgccgaggg ccgccactcc accggcgcca aggacgagct
      781 gtaagaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct agataa
//
