LOCUS       sequence-154562-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically 
            selected. Modified from BBa_I13453. Has near identical expression as
            36INS1-pBAD_AB [F:36INS2-pBAD:B].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Carr SB, Beal J, Densmore DM
  TITLE     Reducing DNA context dependence in bacterial promoters.
  JOURNAL   PLoS One. 2017 Apr 19;12(4):e0176013. doi: 
            10.1371/journal.pone.0176013. eCollection 2017.
  PUBMED    28422998
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2017-04-19"; volume: "12(4):e0176013. doi"; pages: " 
            10.1371/journal.pone.0176013. eCollection 2017"
COMMENT     Sequence Label: 36INS2-pBAD_FB
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     87..104
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    110..130
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(219..238)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     misc_feature    326..347
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      350..393
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(397..415)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        483..587
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
       61 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcc tgcagcggcc
      121 gctactagta ggtctctagt agctagccca aaaaaacggt atggagaaac agtagagagt
      181 tgcgataaaa agcgtcaggt aggatccgct aatcttatgg ataaaaatgc tatggcatag
      241 caaagtgtga cgccgtgcaa ataatcaatg ttagaagctg accaaccata cgcagaaaag
      301 cgtgctaagc gtgagaccca ctagtctcta gaagcggccg cgaattccag aaatcatcct
      361 tagcgaaagc taaggatttt ttttatctga aattctgcct cgtgatacgc ctatttttat
      421 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      481 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      541 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      601 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      661 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      721 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      781 caatgatgag cacttttaa
//
