LOCUS       sequence-154570-        1835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1835)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Sec61-N-18
FEATURES             Location/Qualifiers
     source          1..1835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..323
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     CDS             378..1055
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1104..1781
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
ORIGIN
        1 gcagctagtg aaccgtcaga tccgctagcg ccaccatgcc tggtccgacc cccagtggca
       61 ctaacgtggg atcctcaggg cgctctccca gcaaagcagt ggccgcccgg gcggcgggat
      121 ccactgtccg gcagaggaaa aatgccagct gtgggacaag gagtgcaggc cgcacaacct
      181 cggcaggcac cggggggatg tggcgattct acacagaaga ttcacctggg ctcaaagttg
      241 gccctgttcc agtattggtt atgagtcttc tgttcatcgc ttctgtattt atgttgcaca
      301 tttggggcaa gtacactcgt tcgggacgaa ttctgcagtc gacggtaccg cgggcccggg
      361 atccaccggt cgccaccatg agtgcgatta agccagacat gaagatcaac ctccgtatgg
      421 aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc aagccttttg
      481 agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct tttgcctttg
      541 atatcctgac cactgcattc cattacggca acagggtatt cgccgaatat ccagaccaca
      601 tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga agcttgactt
      661 tcgaagacgg gggcatttgc attgccagaa acgacataac aatggaaggg gacactttct
      721 ataataaagt tcgatttcac ggtgtaaact ttcccgccaa tggtccagtt atgcagaaga
      781 agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga gtgctgactg
      841 gtgatattcg catggctttg ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa
      901 ctacttacaa agctaaggag aagggtgtca agttaccagg ctaccacttt gtggaccact
      961 gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat gagcatgctg
     1021 ttgctcattc tggattgcct gacaatgcca gacgatccgg tagcggtcac ggtactggtt
     1081 ctactggttc tggttcttct gagagtgcga ttaagccaga catgaagatc aacctccgta
     1141 tggaaggcaa cgtaaacggg caccactttg tgatcgacgg agatggtaca ggcaagcctt
     1201 ttgagggaaa acagagtatg gatcttgaag tcaaagaggg cggacctctg ccttttgcct
     1261 ttgatatcct gaccactgca ttccattacg gcaacagggt attcgccgaa tatccagacc
     1321 acatacaaga ctattttaag cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga
     1381 ctttcgaaga cgggggcatt tgcattgcca gaaacgacat aacaatggaa ggggacactt
     1441 tctataataa agttcgattt cacggtgtaa actttcccgc caatggtcca gttatgcaga
     1501 agaagacgct gaaatgggag ccctccactg agaaaatgta tgtgcgtgat ggagtgctga
     1561 ctggtgatat tcgcatggct ttgttgcttg aaggaaatgc ccattaccga tgtgacttca
     1621 gaactactta caaagctaag gagaagggtg tcaagttacc aggctaccac tttgtggacc
     1681 actgcattga gattttaagc catgacaaag attacaacaa ggttaagctg tatgagcatg
     1741 ctgttgctca ttctggattg cctgacaatg ccagacgata agcggccgcg actctagatc
     1801 ataatcagcc ataccacatt gtagaagatt gccac
//
