LOCUS       sequence-154698-        1179 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing TP53 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TP53_p.S215I
FEATURES             Location/Qualifiers
     source          1..1179
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1179
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLML
                     SPDDIEQWFTEDPGPDEAPRMPEAAPRVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQ
                     GSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIY
                     KQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHIVVVPYEP
                     PEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACAGRD
                     RRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM
                     FRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
ORIGIN
        1 atggaggagc cgcagtcaga tcctagcgtc gagccccctc tgagtcagga aacattttca
       61 gacctatgga aactacttcc tgaaaacaac gttctgtccc ccttgccgtc ccaagcaatg
      121 gatgatttga tgctgtcccc ggacgatatt gaacaatggt tcactgaaga cccaggtcca
      181 gatgaagctc ccagaatgcc agaggctgct ccccgcgtgg cccctgcacc agcagctcct
      241 acaccggcgg cccctgcacc agccccctcc tggcccctgt catcttctgt cccttcccag
      301 aaaacctacc agggcagcta cggtttccgt ctgggcttct tgcattctgg gacagccaag
      361 tctgtgactt gcacgtactc ccctgccctc aacaagatgt tttgccaact ggccaagacc
      421 tgccctgtgc agctgtgggt tgattccaca cccccgcccg gcacccgcgt ccgcgccatg
      481 gccatctaca agcagtcaca gcacatgacg gaggttgtga ggcgctgccc ccaccatgag
      541 cgctgctcag atagcgatgg tctggcccct cctcagcatc ttatccgagt ggaaggaaat
      601 ttgcgtgtgg agtatttgga tgacagaaac acttttcgac atattgtggt ggtgccctat
      661 gagccgcctg aggttggctc tgactgtacc accatccact acaactacat gtgtaacagt
      721 tcctgcatgg gcggcatgaa ccggaggccc atcctcacca tcatcacact ggaagactcc
      781 agtggtaatc tactgggacg gaacagcttt gaggtgcgtg tttgtgcctg tgctgggaga
      841 gaccggcgca cagaggaaga gaatctccgc aagaaagggg agcctcacca cgagctgccc
      901 ccagggagca ctaagcgagc actgcccaac aacaccagct cctctcccca gccaaagaag
      961 aaaccactgg atggagaata tttcaccctt cagatccgtg ggcgtgagcg cttcgagatg
     1021 ttccgagagc tgaatgaggc cttggaactc aaggatgccc aggctgggaa ggagccaggg
     1081 gggagcaggg ctcactccag ccacctgaag tccaaaaagg gtcagtctac ctcccgccat
     1141 aaaaaactca tgttcaagac agaagggcct gactcagac
//
