LOCUS       sequence-154772-        1437 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing AKT1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_AKT1_p.V201I
FEATURES             Location/Qualifiers
     source          1..1437
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1437
                     /codon_start=1
                     /gene="AKT1"
                     /product="human serine/threonine protein kinase activated 
                     by growth factors"
                     /label=Akt1
                     /translation="MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDV
                     DQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQ
                     TVADGLKKQEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGKV
                     ILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRILQNSRHPFLTALKYSFQTHDRL
                     CFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLML
                     DKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYE
                     MMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDAK
                     EIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSME
                     CVDSERRPHFPQFSYSASGTA"
ORIGIN
        1 atgagcgacg tggctattgt gaaggagggt tggctgcaca aacgagggga gtacatcaag
       61 acctggcggc cacgctactt cctcctcaag aatgatggca ccttcattgg ctacaaggag
      121 cggccgcagg atgtggacca acgtgaggct cccctcaaca acttctctgt ggcgcagtgc
      181 cagctgatga agacggagcg gccccggccc aacaccttca tcatccgctg cctgcagtgg
      241 accactgtca tcgaacgcac cttccatgtg gagactcctg aggagcggga ggagtggaca
      301 accgccatcc agactgtggc tgacggcctc aagaagcagg aggaggagat ggacttccgg
      361 tcgggctcac ccagtgacaa ctcaggggct gaagagatgg aggtgtccct ggccaagccc
      421 aagcaccgcg tgaccatgaa cgagtttgag tacctgaagc tgctgggcaa gggcactttc
      481 ggcaaggtga tcctggtgaa ggagaaggcc acaggccgct actacgccat gaagatcctc
      541 aagaaggaag tcatcgtggc caaggacgag gtggcccaca cactcaccga gaaccgcatc
      601 ctgcagaact ccaggcaccc cttcctcaca gccctgaagt actctttcca gacccacgac
      661 cgcctctgct ttgtcatgga gtacgccaac gggggcgagc tgttcttcca cctgtcccgg
      721 gagcgtgtgt tctccgagga ccgggcccgc ttctatggcg ctgagattgt gtcagccctg
      781 gactacctgc actcggagaa gaacgtggtg taccgggacc tcaagctgga gaacctcatg
      841 ctggacaagg acgggcacat taagatcaca gacttcgggc tgtgcaagga ggggatcaag
      901 gacggtgcca ccatgaagac cttttgcggc acacctgagt acctggcccc cgaggtgctg
      961 gaggacaatg actacggccg tgcagtggac tggtgggggc tgggcgtggt catgtacgag
     1021 atgatgtgcg gtcgcctgcc cttctacaac caggaccatg agaagctttt tgagctcatc
     1081 ctcatggagg agatccgctt cccgcgcacg cttggtcccg aggccaagtc cttgctttca
     1141 gggctgctca agaaggaccc caagcagagg cttggcgggg gctccgagga cgccaaggag
     1201 atcatgcagc atcgcttctt tgccggtatc gtgtggcagc acgtgtacga gaagaagctc
     1261 agcccaccct tcaagcccca ggtcacgtcg gagactgaca ccaggtattt tgatgaggag
     1321 ttcacggccc agatgatcac catcacacca cctgaccaag atgacagcat ggagtgtgtg
     1381 gacagcgagc gcaggcccca cttcccccag ttctcctact cggccagcgg cacggcc
//
