LOCUS       sequence-154775-        1440 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing AKT1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_AKT1_p.D44N
FEATURES             Location/Qualifiers
     source          1..1440
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1440
                     /codon_start=1
                     /gene="AKT1"
                     /product="human serine/threonine protein kinase activated 
                     by growth factors"
                     /label=Akt1
                     /translation="MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQNV
                     DQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQ
                     TVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGK
                     VILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDR
                     LCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLM
                     LDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMY
                     EMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDA
                     KEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSM
                     ECVDSERRPHFPQFSYSASGTA"
ORIGIN
        1 atgagcgacg tggctattgt gaaggagggt tggctgcaca aacgagggga gtacatcaag
       61 acctggcggc cacgctactt cctcctcaag aatgatggca ccttcattgg ctacaaggag
      121 cggccgcaga atgtggacca acgtgaggct cccctcaaca acttctctgt ggcgcagtgc
      181 cagctgatga agacggagcg gccccggccc aacaccttca tcatccgctg cctgcagtgg
      241 accactgtca tcgaacgcac cttccatgtg gagactcctg aggagcggga ggagtggaca
      301 accgccatcc agactgtggc tgacggcctc aagaagcagg aggaggagga gatggacttc
      361 cggtcgggct cacccagtga caactcaggg gctgaagaga tggaggtgtc cctggccaag
      421 cccaagcacc gcgtgaccat gaacgagttt gagtacctga agctgctggg caagggcact
      481 ttcggcaagg tgatcctggt gaaggagaag gccacaggcc gctactacgc catgaagatc
      541 ctcaagaagg aagtcatcgt ggccaaggac gaggtggccc acacactcac cgagaaccgc
      601 gtcctgcaga actccaggca ccccttcctc acagccctga agtactcttt ccagacccac
      661 gaccgcctct gctttgtcat ggagtacgcc aacgggggcg agctgttctt ccacctgtcc
      721 cgggagcgtg tgttctccga ggaccgggcc cgcttctatg gcgctgagat tgtgtcagcc
      781 ctggactacc tgcactcgga gaagaacgtg gtgtaccggg acctcaagct ggagaacctc
      841 atgctggaca aggacgggca cattaagatc acagacttcg ggctgtgcaa ggaggggatc
      901 aaggacggtg ccaccatgaa gaccttttgc ggcacacctg agtacctggc ccccgaggtg
      961 ctggaggaca atgactacgg ccgtgcagtg gactggtggg ggctgggcgt ggtcatgtac
     1021 gagatgatgt gcggtcgcct gcccttctac aaccaggacc atgagaagct ttttgagctc
     1081 atcctcatgg aggagatccg cttcccgcgc acgcttggtc ccgaggccaa gtccttgctt
     1141 tcagggctgc tcaagaagga ccccaagcag aggcttggcg ggggctccga ggacgccaag
     1201 gagatcatgc agcatcgctt ctttgccggt atcgtgtggc agcacgtgta cgagaagaag
     1261 ctcagcccac ccttcaagcc ccaggtcacg tcggagactg acaccaggta ttttgatgag
     1321 gagttcacgg cccagatgat caccatcaca ccacctgacc aagatgacag catggagtgt
     1381 gtggacagcg agcgcaggcc ccacttcccc cagttctcct actcggccag cggcacggcc
//
