LOCUS       sequence-154858-        4074 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing PIK3R4 ,part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PIK3R4_p.E1209K
FEATURES             Location/Qualifiers
     source          1..4074
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgggaaatc agcttgctgg cattgctccc tcccagatcc tttctgtaga gagttatttt
       61 tcagatattc atgactttga atatgataaa agcctgggga gtactcggtt ttttaaagtt
      121 gctcgagcca agcaccgaga aggcctggtc gttgtgaagg tttttgcaat tcaggatccc
      181 acattgcctt taaccagcta taaacaagag ctggaggaac tgaaaatcag gcttaattct
      241 gcacagaatt gtctaccttt ccagaaagca tcagaaaaag catctgagaa agcagctatg
      301 ctctttaggc agtatgtgcg agacaatctc tatgatcgca tcagtacccg tccattcttg
      361 aataacattg agaagcgctg gattgctttc cagatcctga cagctgtgga ccaagcacac
      421 aaatctggag ttcgtcatgg ggacatcaag actgagaatg tgatggtcac cagttggaat
      481 tgggttcttc taactgattt tgccagtttt aagcccactt atcttccaga agacaacccg
      541 gcagatttca attatttctt tgacacatca cggaggagaa cttgctatat tgctcctgaa
      601 cgttttgttg atggtgggat gtttgccact gagttagaat atatgagaga tccttcaact
      661 ccgcttgtag acttaaatag caatcagaga agaagaggag agttgaagag agcaatggac
      721 atcttttcag caggttgtgt gatagctgag ctttttacag aaggtgtacc attatttgat
      781 ctctctcaac ttttggctta tagaaatgga cattttttcc ctgaacaagt gctaaataaa
      841 attgaagatc acagtatcag agaattggta actcagatga ttcaccgtga gccagataaa
      901 cgtttagagg cagaagatta cttaaaacag cagcgtggca atgcctttcc tgaaatattt
      961 tacacttttc ttcagcccta catggcccag tttgccaagg aaacgtttct ttctgcagat
     1021 gagcgtattc tggttatacg gaaggatttg ggcaacatta ttcacaatct ctgtggacat
     1081 gatctgccag aaaaagccga aggagagcct aaggaaaatg ggctggttat cttggtatct
     1141 gttataacat cctgcctaca gacccttaaa tactgtgatt ccaaactagc tgctttggaa
     1201 ctgattcttc atttggctcc aagattaagt gttgaaatcc ttttggatcg tattactcca
     1261 tatcttttgc atttcagcaa tgactctgtt cctagggtga gggctgaagc cttgaggacg
     1321 ttgaccaaag ttcttgctct cgtcaaagag gttcctcgta atgatatcaa tatttatccg
     1381 gaatacattc tgccaggcat agcccactta gcccaagatg atgctactat cgttagacta
     1441 gcctatgctg aaaacatagc tctgctggca gaaacagctc tgagattcct ggaattagta
     1501 cagttaaaaa atcttaatat ggaaaatgac cccaataatg aagaaataga tgaggttaca
     1561 catccaaatg gaaattatga cacagagctc caagccttac atgaaatggt ccagcagaaa
     1621 gttgttactt tgctaagtga ccctgaaaat attgtaaaac aaaccttgat ggaaaatgga
     1681 ataacacggc tgtgtgtatt ctttggacgt cagaaagcca acgatgtttt gttgtcccac
     1741 atgattactt tcctaaatga taagaatgat tggcatctac gtggagcatt ttttgatagt
     1801 atagttggtg ttgctgccta tgttggctgg caaagctcct caattctcaa gcctctgctg
     1861 caacaaggtc ttagtgatgc tgaggaattt gtcattgtga aagctcttta tgcccttact
     1921 tgtatgtgcc agttaggact gctacaaaaa ccccatgttt acgaatttgc cagtgatatt
     1981 gcccccttcc tgtgtcatcc caatttatgg atacgttatg gtgccgtggg atttatcaca
     2041 gtggtagctc gtcaaataag tacagctgat gtctactgta aactgatgcc ttatcttgac
     2101 ccatatatta cccaaccaat aatacagatt gaaagaaaac ttgttctgct cagtgtttta
     2161 aaggaaccag taagtcgttc tatatttgat tatgctttga ggtctaaaga tattactagc
     2221 ttgttcagac atcttcacat gcgtcagaag aaacgaaatg gttctcttcc cgactgccct
     2281 ccgccagagg atcctgccat agcacagctt ctgaagaagt tgctctcaca gggaatgaca
     2341 gaggaagagg aagacaaact tctggcactg aaagacttca tgatgaaatc taataaagca
     2401 aaggccaata tagtggacca gagccatctt catgatagta gtcagaaagg tgtaattgac
     2461 ttggcagctt taggcataac tgggagacaa gttgatcttg ttaaaaccaa acaagaacca
     2521 gatgacaaac gggccagaaa acatgtaaaa caagactcaa atgtaaatga agaatggaaa
     2581 agcatgtttg ggtcactgga cccaccaaac atgccacagg ccctacctaa agggagtgat
     2641 caggaggtga ttcagactgg gaaacctcct cgttccgagt cctctgctgg catttgtgtc
     2701 cctttgtcaa cttcttcaca ggttccagaa gtgacaactg tccaaaataa aaaaccagta
     2761 ataccggttt taagtagtac aatcttacca tccacctatc agattcgaat tacaacttgt
     2821 aaaactgaac ttcagcaact catccagcaa aagcgggagc agtgcaatgc tgagagaata
     2881 gctaagcaga tgatggaaaa tgctgaatgg gagagtaaac caccaccacc tggatggcgt
     2941 cctaaagggc tgttagttgc ccatcttcat gagcataaat ctgctgtgaa tcgaattaga
     3001 gtctctgatg aacactcact ttttgcaaca tgttcaaatg atggcacagt gaaaatctgg
     3061 aacagtcaaa agatggaggg gaagaccacc actaccagat ctattcttac atacagccga
     3121 attggaggac gagtcaagac gctcacattc tgccaaggct cccactattt agccatagca
     3181 tctgataatg gtgctgtcca gcttcttgga attgaggctt ctaagctgcc caagtctcct
     3241 aaaatccatc ctctacaaag cagaattcta gatcagaagg aggacggttg tgttgtggat
     3301 atgcatcact tcaactctgg agcacagtct gttcttgcct atgccactgt gaatggctct
     3361 ctggttggct gggaccttag gtcttcaagc aatgcgtgga ctttaaagca tgatttaaag
     3421 tcgggcctca tcacttcctt tgctgtggac atccaccaat gctggctctg cattggtaca
     3481 agcagtggta ccatggcttg ttgggacatg aggttccagt tgccaatttc aagtcactgt
     3541 catccttcca gggctcgaat cagacgcctc tcaatgcacc ctctgtatca gtcctgggtg
     3601 attgcagctg ttcagggcaa caacaaagtg tccatgtggg acatggagac tggtgacaga
     3661 agatttactc tctgggccag cagtgcacca ccactttctg aattacagcc ttctcctcat
     3721 agcgtccatg gtatctactg tagtcctgca gatggaaatc ctatcctact aacagctggc
     3781 tcagatatga aaataaggtt ttgggacttg gcttacccag aaaggtccta tgttgttgca
     3841 ggaagtacta gttccccatc tgtgtcctac tacaggaaaa taattgaagg cactgaagtt
     3901 gtccaggaaa ttcagaataa gcagaaagta ggaccaagtg atgacacccc tcgaaggggc
     3961 ccagagtccc tgcccgtggg acatcatgac atcatcactg atgtcgccac attccagacc
     4021 acacagggct tcatcgtaac tgcttctaga gatgggattg tgaaggtgtg gaaa
//
