LOCUS       sequence-154860-        2724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing GEN1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_GEN1_p.L235R
FEATURES             Location/Qualifiers
     source          1..2724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgggagtga atgacttgtg gcaaattttg gagcctgtta agcaacacat ccccttgcgt
       61 aatcttggtg ggaaaaccat tgcagttgat ctgagtctct gggtgtgtga ggcacagaca
      121 gtcaaaaaaa tgatgggcag cgtcatgaag ccccacctca ggaacttatt ttttcgtatc
      181 tcatatttaa cacaaatgga tgtaaaactg gtatttgtta tggaagggga accaccaaag
      241 ctgaaagctg atgtcataag caagaggaat cagactcggt atgggtcttc tggaaaatcg
      301 tggtctcaga aaacagggag atcacatttt aaatcagtct taagagagtg cctccatatg
      361 ctcgaatgct taggaatccc ctgggttcag gctgctgggg aagctgaagc catgtgtgct
      421 tatctcaatg ctggtggtca tgtcgatggc tgcctcacca atgatggaga tactttcctt
      481 tatggggccc agactgttta caggaatttc actatgaata caaaggaccc acatgttgac
      541 tgttacacaa tgtcatctat caagagtaaa ctaggtttgg atagagatgc tctggttgga
      601 ttagcaatac ttcttggctg tgattatctc ccaaagggag tccctggagt tggaaaagag
      661 caagcattaa aacttataca gattttgaaa gggcaaagtt tacgtcagag gtttaatcgg
      721 tggaatgaaa catcttgtaa ctctagtcca caactgctag tcactaaaaa actggctcat
      781 tgttccgtat gttcccatcc aggttcacct aaggatcatg aacgtaatgg atgcagatta
      841 tgtaaaagtg ataaatattg tgagccacat gactatgaat actgctgtcc ttgtgagtgg
      901 caccgtacag aacatgatag gcaactcaat gaagtagaga acaatattaa gaagaaagct
      961 tgctgttgtg agggattccc attccatgag gttattcaag aattcctttt aaacaaggat
     1021 aaattggtga aggttatcag gtaccaaaga cctgatttgt tattgtttca gagatttact
     1081 cttgaaaaaa tggagtggcc caatcactat gcatgtgaga aattgctggt acttttgacc
     1141 cattatgaca tgatagaaag aaagcttggt agcagaaact ctaatcaact acagccaatt
     1201 cgaattgtta agactcgaat cagaaatgga gttcattgtt ttgaaataga atgggaaaag
     1261 cctgaacatt atgctatgga agataaacaa catggagaat ttgctttatt aacaattgag
     1321 gaagaatcat tgtttgaagc agcatatcct gagatcgttg ctgtttacca aaaacaaaag
     1381 ttagaaatta aagggaagaa acaaaaacgt attaagccta aagaaaacaa tttgccagaa
     1441 ccagatgaag taatgagctt tcagtcacac atgactttaa aacccacatg tgaaatcttt
     1501 cataagcaga attccaagtt aaattcgggg atttcccctg atcctacatt accacaggaa
     1561 tctatttctg cctcattgaa tagcttgctt ttacctaaaa atactccatg tttgaatgca
     1621 caagaacagt tcatgtcttc tctaagacct ttggctatac agcaaattaa agctgtcagt
     1681 aagtctctaa tttcagaatc tagtcaaccc aatacctcat ctcataatat atccgtgatt
     1741 gctgatctac acttgagcac tattgactgg gaaggtactt cttttagtaa ttctccagct
     1801 attcaaagga atactttttc tcatgattta aaatcagaag ttgaatcaga gctatcagcc
     1861 atccctgatg gctttgaaaa tatcccagaa caactgtcct gtgaatcaga aaggtacact
     1921 gcaaacataa agaaagtgtt ggatgaggat tctgatggga ttagtcctga agagcatcta
     1981 ctttctggca ttactgattt atgtcttcag gatttgcctt taaaggaacg aatatttaca
     2041 aaattatcat atcctcagga taatctacaa ccagatgtca acctgaaaac tttgtccata
     2101 cttagtgtaa aagaatcttg tattgctaac agtggttctg attgtacatc acatctttca
     2161 aaggatcttc caggaattcc cttgcaaaat gaatccagag actctaaaat tctaaaagga
     2221 gaccagctgc ttcaagaaga ctataaagtc aatacttctg tcccttattc tgtcagtaac
     2281 acagtggtaa agacctgcaa tgttagacca ccaaatactg ctttagatca tagtagaaaa
     2341 gttgatatgc aaaccactcg gaaaatttta atgaagaaga gtgtttgcct tgacagacat
     2401 tcctctgatg aacaaagtgc cccagtgttt gggaaagcta agtacacaac tcaaagaatg
     2461 aagcacagtt ctcaaaagca taattcatcc catttcaaag aaagtggcca taacaagttg
     2521 agtagcccta agatacatat taaagaaact gaacagtgtg tcagatctta tgaaacagct
     2581 gaaaatgaag aaagctgttt cccagattca acaaaaagtt ctctgagttc tctacaatgt
     2641 cataagaaag aaaacaactc tggtacttgt ttggatagcc ctcttccttt acgccagaga
     2701 ttaaaactaa gattccaaag cact
//
