LOCUS       sequence-154864-        2736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing DNMT3A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_DNMT3A_p.G746V
FEATURES             Location/Qualifiers
     source          1..2736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..2736
                     /codon_start=1
                     /product="DNA methyltransferase 3A"
                     /label=DNMT3A
                     /translation="MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTA
                     RKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEG
                     SPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIESMKM
                     EGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIA
                     GMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATKAGDDE
                     PEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVC
                     VEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPVCHDSDESDTAKAV
                     EVQNKPMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPR
                     KSTAEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFVGGMCQNC
                     KNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGAAQAAI
                     KEDPWNCYMCGHKGTYGLLRRREDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIR
                     VLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKH
                     IQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEVDDRPFFW
                     LFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDK
                     LELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFP
                     VHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV"
ORIGIN
        1 atgcccgcca tgccctccag cggccccggg gacaccagca gctctgctgc ggagcgggag
       61 gaggaccgaa aggacggaga ggagcaggag gagccgcgtg gcaaggagga gcgccaagag
      121 cccagcacca cggcacggaa ggtggggcgg cctgggagga agcgcaagca ccccccggtg
      181 gaaagcggtg acacgccaaa ggaccctgcg gtgatctcca agtccccatc catggcccag
      241 gactcaggcg cctcagagct attacccaat ggggacttgg agaagcggag tgagccccag
      301 ccagaggagg ggagccctgc tggggggcag aagggcgggg ccccagcaga gggagagggt
      361 gcagctgaga ccctgcctga agcctcaaga gcagtggaaa atggctgctg cacccccaag
      421 gagggccgag gagcccctgc agaagcgggc aaagaacaga aggagaccaa catcgaatcc
      481 atgaaaatgg agggctcccg gggccggctg cggggtggct tgggctggga gtccagcctc
      541 cgtcagcggc ccatgccgag gctcaccttc caggcggggg acccctacta catcagcaag
      601 cgcaagcggg acgagtggct ggcacgctgg aaaagggagg ctgagaagaa agccaaggtc
      661 attgcaggaa tgaatgctgt ggaagaaaac caggggcccg gggagtctca gaaggtggag
      721 gaggccagcc ctcctgctgt gcagcagccc actgaccccg catcccccac tgtggctacc
      781 acgcctgagc ccgtggggtc cgatgctggg gacaagaatg ccaccaaagc aggcgatgac
      841 gagccagagt acgaggacgg ccggggcttt ggcattgggg agctggtgtg ggggaaactg
      901 cggggcttct cctggtggcc aggccgcatt gtgtcttggt ggatgacggg ccggagccga
      961 gcagctgaag gcacccgctg ggtcatgtgg ttcggagacg gcaaattctc agtggtgtgt
     1021 gttgagaagc tgatgccgct gagctcgttt tgcagtgcgt tccaccaggc cacgtacaac
     1081 aagcagccca tgtaccgcaa agccatctac gaggtcctgc aggtggccag cagccgcgcg
     1141 gggaagctgt tcccggtgtg ccacgacagc gatgagagtg acactgccaa ggccgtggag
     1201 gtgcagaaca agcccatgat tgaatgggcc ctggggggct tccagccttc tggccctaag
     1261 ggcctggagc caccagaaga agagaagaat ccctacaaag aagtgtacac ggacatgtgg
     1321 gtggaacctg aggcagctgc ctacgcacca cctccaccag ccaaaaagcc ccggaagagc
     1381 acagcggaga agcccaaggt caaggagatt attgatgagc gcacaagaga gcggctggtg
     1441 tacgaggtgc ggcagaagtg ccggaacatt gaggacatct gcatctcctg tgggagcctc
     1501 aatgttaccc tggaacaccc cctcttcgtt ggaggaatgt gccaaaactg caagaactgc
     1561 tttctggagt gtgcgtacca gtacgacgac gacggctacc agtcctactg caccatctgc
     1621 tgtgggggcc gtgaggtgct catgtgcgga aacaacaact gctgcaggtg cttttgcgtg
     1681 gagtgtgtgg acctcttggt ggggccgggg gctgcccagg cagccattaa ggaagacccc
     1741 tggaactgct acatgtgcgg gcacaagggt acctacgggc tgctgcggcg gcgagaggac
     1801 tggccctccc ggctccagat gttcttcgct aataaccacg accaggaatt tgaccctcca
     1861 aaggtttacc cacctgtccc agctgagaag aggaagccca tccgggtgct gtctctcttt
     1921 gatggaatcg ctacagggct cctggtgctg aaggacttgg gcattcaggt ggaccgctac
     1981 attgcctcgg aggtgtgtga ggactccatc acggtgggca tggtgcggca ccaggggaag
     2041 atcatgtacg tcggggacgt ccgcagcgtc acacagaagc atatccagga gtggggccca
     2101 ttcgatctgg tgattggggg cagtccctgc aatgacctct ccatcgtcaa ccctgctcgc
     2161 aagggcctct acgagggcac tggccggctc ttctttgagt tctaccgcct cctgcatgat
     2221 gcgcggccca aggaggtaga tgatcgcccc ttcttctggc tctttgagaa tgtggtggcc
     2281 atgggcgtta gtgacaagag ggacatctcg cgatttctcg agtccaaccc tgtgatgatt
     2341 gatgccaaag aagtgtcagc tgcacacagg gcccgctact tctggggtaa ccttcccggt
     2401 atgaacaggc cgttggcatc cactgtgaat gataagctgg agctgcagga gtgtctggag
     2461 catggcagga tagccaagtt cagcaaagtg aggaccatta ctacgaggtc aaactccata
     2521 aagcagggca aagaccagca ttttcctgtc ttcatgaatg agaaagagga catcttatgg
     2581 tgcactgaaa tggaaagggt atttggtttc ccagtccact atactgacgt ctccaacatg
     2641 agccgcttgg cgaggcagag actgctgggc cggtcatgga gcgtgccagt catccgccac
     2701 ctcttcgctc cgctgaagga gtattttgcg tgtgtg
//
