LOCUS       sequence-154871-        1924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing THEMIS , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_THEMIS_p.D240N
FEATURES             Location/Qualifiers
     source          1..1924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcattat cactggaaga attcgtccac tcccttgacc tcaggaccct acccagggtt
       61 ctagaaatcc aggcaggcat ctatcttgaa ggctctattt atgaaatgtt tggaaatgaa
      121 tgctgttttt caacaggaga agtgattaaa attactggtc tcaaagttaa gaagatcata
      181 gctgaaattt gtgagcagat tgaaggttgt gagtctctac agccatttga actgcctatg
      241 aattttccag gtctttttaa gattgtggct gataaaactc cataccttac tatggaagaa
      301 atcacaagga ccattcatat tggaccaagt agactagggc atccttgctt ctatcatcag
      361 aaggatataa aactagagaa cctcatcata aagcagggtg agcaaatcat gctcaactca
      421 gttgaagaga ttgatggaga aataatggtg agctgtgcag tagcaaggaa tcatcaaact
      481 cactcattta atttgccttt gtcacaagaa ggagaattct acgagtgtga agatgaacgt
      541 atttacactc taaaggagat tgttgaatgg aagattccta agaacagaac aagaactgta
      601 aaccttacag atttttcaaa taagtgggac tcaacgaatc catttcctaa agacttttat
      661 ggtaccctga ttctcaagcc tgtttatgaa attcaaggtg tgatgaaatt tcgaaaaaat
      721 ataatccgca tcctccccag tctagatgtc gaagtcaaag acatcactga ttcttacgat
      781 gctaactggt ttcttcagct gttatcaaca gaagatcttt ttgaaatgac tagtaaagag
      841 ttccccatag tgactgaagt catagaagca cctgaaggaa accacctgcc ccaaagcatt
      901 ttacagcctg ggaaaaccat tgtgatccac aaaaagtacc aggcatcaag aatcttagct
      961 tcagaaatta gaagcaattt tcctaaaaga cacttcttga tccccactag ctataaaggc
     1021 aagttcaagc ggcgaccgag ggagttccca acggcctatg acctagagat cgctaagagt
     1081 gaaaaggagc ctcttcacgt ggtggccacc aaagcgtttc attcccctca tgacaagctg
     1141 tcatccgtat ctgttgggga ccagtttctg gtgcatcagt cagagacgac tgaagtcctc
     1201 tgtgagggaa taaaaaaagt ggtgaatgtt ctggcctgtg aaaaaatcct caaaaagtcc
     1261 tatgaggctg cgctgctccc tttgtacatg gaaggaggtt ttgtagaggt gattcatgat
     1321 aagaaacagt acccgatttc tgagctctgt aaacagttcc gtttgccctt caatgtgaaa
     1381 ggtgtctgtc agggatcttt ccattgaaga ggacgtgttg gctgccacac caggactgca
     1441 gttggaggag gacattacag actcttacct actcataagt gactttgcca accccacgga
     1501 gtgctgggaa attcctgtgg gccgcttgaa tatgactgtt cagttagtta gtaatttctc
     1561 tagggatgca gaaccatttc tagtcaggac tctggtagaa gagatcactg aagagcaata
     1621 ttacatgatg cggagatatg aaagctcagc ctcacatccc ccacctcgcc ctccgaaaca
     1681 cccctcagta gaggaaacaa agttaaccct gctaacctta gcagaagaaa ggacggtaga
     1741 cctgcccaag tctcccaagc gtcatcacgt agacataacc aagaaacttc acccaaatca
     1801 agctggcctg gattcaaaag tactgattgg tagtcagaat gatttggtgg atgaagagaa
     1861 agaaaggagc aaccgtgggg ccacagcagt agcagaaaca ttcaaaaatg aaaaacatca
     1921 aaaa
//
