LOCUS       sequence-154879-        1923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing THEMIS , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_THEMIS_p.H366Q
FEATURES             Location/Qualifiers
     source          1..1923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcattat cactggaaga attcgtccac tcccttgacc tcaggaccct acccagggtt
       61 ctagaaatcc aggcaggcat ctatcttgaa ggctctattt atgaaatgtt tggaaatgaa
      121 tgctgttttt caacaggaga agtgattaaa attactggtc tcaaagttaa gaagatcata
      181 gctgaaattt gtgagcagat tgaaggttgt gagtctctac agccatttga actgcctatg
      241 aattttccag gtctttttaa gattgtggct gataaaactc cataccttac tatggaagaa
      301 atcacaagga ccattcatat tggaccaagt agactagggc atccttgctt ctatcatcag
      361 aaggatataa aactagagaa cctcatcata aagcagggtg agcaaatcat gctcaactca
      421 gttgaagaga ttgatggaga aataatggtg agctgtgcag tagcaaggaa tcatcaaact
      481 cactcattta atttgccttt gtcacaagaa ggagaattct acgagtgtga agatgaacgt
      541 atttacactc taaaggagat tgttgaatgg aagattccta agaacagaac aagaactgta
      601 aaccttacag atttttcaaa taagtgggac tcaacgaatc catttcctaa agacttttat
      661 ggtaccctga ttctcaagcc tgtttatgaa attcaaggtg tgatgaaatt tcgaaaagat
      721 ataatccgca tcctccccag tctagatgtc gaagtcaaag acatcactga ttcttacgat
      781 gctaactggt ttcttcagct gttatcaaca gaagatcttt ttgaaatgac tagtaaagag
      841 ttccccatag tgactgaagt catagaagca cctgaaggaa accacctgcc ccaaagcatt
      901 ttacagcctg ggaaaaccat tgtgatccac aaaaagtacc aggcatcaag aatcttagct
      961 tcagaaatta gaagcaattt tcctaaaaga cacttcttga tccccactag ctataaaggc
     1021 aagttcaagc ggcgaccgag ggagttccca acggcctatg acctagagat cgctaagagt
     1081 gaaaaggagc ctcttcaggt ggtggccacc aaagcgtttc attcccctca tgacaagctg
     1141 tcatccgtat ctgttgggga ccagtttctg gtgcatcagt cagagacgac tgaagtcctc
     1201 tgtgagggaa taaaaaaagt ggtgaatgtt ctggcctgtg aaaaaatcct caaaaagtcc
     1261 tatgaggctg cgctgctccc tttgtacatg gaaggaggtt ttgtagaggt gattcatgat
     1321 aagaaacagt acccgatttc tgagctctgt aaacagttcc gtttgccctt caatgtgaag
     1381 gtgtctgtca gggatctttc cattgaagag gacgtgttgg ctgccacacc aggactgcag
     1441 ttggaggagg acattacaga ctcttaccta ctcataagtg actttgccaa ccccacggag
     1501 tgctgggaaa ttcctgtggg ccgcttgaat atgactgttc agttagttag taatttctct
     1561 agggatgcag aaccatttct agtcaggact ctggtagaag agatcactga agagcaatat
     1621 tacatgatgc ggagatatga aagctcagcc tcacatcccc cacctcgccc tccgaaacac
     1681 ccctcagtag aggaaacaaa gttaaccctg ctaaccttag cagaagaaag gacggtagac
     1741 ctgcccaagt ctcccaagcg tcatcacgta gacataacca agaaacttca cccaaatcaa
     1801 gctggcctgg attcaaaagt actgattggt agtcagaatg atttggtgga tgaagagaaa
     1861 gaaaggagca accgtggggc cacagcagta gcagaaacat tcaaaaatga aaaacatcaa
     1921 aaa
//
