LOCUS       sequence-154949-        3306 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing PIK3CG , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PIK3CG_p.W212C
FEATURES             Location/Qualifiers
     source          1..3306
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2907..2918
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atggggctgg agaactataa acagcccgtg gtgctgagag aggacaactg ccgaaggcgc
       61 cggaggatga agccgcgcag tgctgcggcc agcctgtcct ccatggagct catccccatc
      121 gagttcgtgc tgcccaccag ccagcgcaaa tgcaagagcc ccgaaacggc gctgctgcac
      181 gtggccggcc acggcaacgt ggagcagatg aaggcccagg tgtggctgcg agcgctggag
      241 accagcgtgg cggcggactt ctaccaccgg ctgggaccgc atcacttcct cctgctctat
      301 cagaagaagg ggcagtggta cgagatctac gacaagtacc aggtggtgca gactctggac
      361 tgcctgcgct actggaaggc cacgcaccgg agcccgggcc agatccacct ggtgcagcgg
      421 cacccgccct ccgaggagtc ccaagccttc cagcggcagc tcacggcgct gattggctat
      481 gacgtcactg acgtcagcaa cgtgcacgac gatgagctgg agttcacgcg ccgtggcttg
      541 gtgaccccgc gcatggcgga ggtggccagc cgcgacccca agctctacgc catgcacccg
      601 tgggtgacgt ccaagcccct cccggagtac ctgtgtaaga agattgccaa caactgcatc
      661 ttcatcgtca ttcaccgcag caccaccagc cagaccatta aggtctcacc cgacgacacc
      721 cccggcgcca tcctgcagag cttcttcacc aagatggcca agaagaaatc tctgatggat
      781 attcccgaaa gccaaagcga acaggatttt gtgctgcgcg tctgtggccg ggatgagtac
      841 ctggtgggcg aaacgcccat caaaaacttc cagtgggtga ggcactgcct caagaacgga
      901 gaagagattc acgtggtact ggacacgcct ccagacccgg ccctagacga ggtgaggaag
      961 gaagagtggc cgctggtgga cgactgcacg ggagtcaccg gctaccatga gcagcttacc
     1021 atccacggca aggaccacga gagtgtgttc accgtgtccc tgtgggactg cgaccgcaag
     1081 ttcagggtca agatcagagg cattgatatc cccgtcctgc ctcggaacac cgacctcaca
     1141 gtttttgtag aggcaaacat ccagcatggg caacaagtcc tttgccaaag gagaaccagc
     1201 cccaaaccct tcacagagga ggtgctgtgg aatgtgtggc ttgagttcag tatcaaaatc
     1261 aaagacttgc ccaaaggggc tctactgaac ctccagatct actgcggtaa agctccagca
     1321 ctgtccagca aggcctctgc agagtccccc agttctgagt ccaagggcaa agttcagctt
     1381 ctctattatg tgaacctgct gctgatagac caccgtttcc tcctgcgccg tggagaatac
     1441 gtcctccaca tgtggcagat atctgggaag ggagaagacc aaggaagctt caatgctgac
     1501 aaactcacgt ctgcaactaa cccagacaag gagaactcaa tgtccatctc cattcttctg
     1561 gacaattact gccacccgat agccctgcct aagcatcagc ccacccctga cccggaaggg
     1621 gaccgggttc gagcagaaat gcccaaccag cttcgcaagc aattggaggc gatcatagcc
     1681 actgatccac ttaaccctct cacagcagag gacaaagaat tgctctggca ttttagatac
     1741 gaaagcctta agcacccaaa agcatatcct aagctattta gttcagtgaa atggggacag
     1801 caagaaattg tggccaaaac ataccaattg ttggccagaa gggaagtctg ggatcaaagt
     1861 gctttggatg ttgggttaac aatgcagctc ctggactgca acttctcaga tgaaaatgta
     1921 agagccattg cagttcagaa actggagagc ttggaggacg atgatgttct gcattacctt
     1981 ctacaattgg tccaggctgt gaaatttgaa ccataccatg atagtgccct tgccagattt
     2041 ctgctgaagc gtggtttaag aaacaaaaga attggtcact ttttgttttg gttcttgaga
     2101 agtgagatag cccagtccag acactatcag cagaggttcg ctgtgattct ggaagcctat
     2161 ctgaggggct gtggcacagc catgctgcac gactttaccc aacaagtcca agtaatcgag
     2221 atgttacaaa aagtcaccct tgatattaaa tcgctctctg ctgaaaagta tgacgtcagt
     2281 tcccaagtta tttcacaact taaacaaaag cttgaaaacc tgcagaattc tcaactcccc
     2341 gaaagcttta gagttccata tgatcctgga ctgaaagcag gagcgctggc aattgaaaaa
     2401 tgtaaagtaa tggcctccaa gaaaaaacca ctatggcttg agtttaaatg tgccgatcct
     2461 acagccctat caaatgaaac aattggaatt atctttaaac atggtgatga tctgcgccaa
     2521 gacatgctta ttttacagat tctacgaatc atggagtcta tttgggagac tgaatctttg
     2581 gatctatgcc tcctgccata tggttgcatt tcaactggtg acaaaatagg aatgatcgag
     2641 attgtgaaag acgccacgac aattgccaaa attcagcaaa gcacagtggg caacacggga
     2701 gcatttaaag atgaagtcct gaatcactgg ctcaaagaaa aatcccctac tgaagaaaag
     2761 tttcaggcag cagtggagag atttgtttat tcctgtgcag gctactgtgt ggcaaccttt
     2821 gttcttggaa taggcgacag acacaatgac aatattatga tcaccgagac aggaaaccta
     2881 tttcatattg acttcgggca cattcttggg aattacaaaa gtttcctggg cattaataaa
     2941 gagagagtgc catttgtgct aacccctgac ttcctctttg tgatgggaac ttctggaaag
     3001 aagacaagcc cacacttcca gaaatttcag gacatctgtg ttaaggctta tctagccctt
     3061 cgtcatcaca caaacctact gatcatcctg ttctccatga tgctgatgac aggaatgccc
     3121 cagttaacaa gcaaagaaga cattgaatat atccgggatg ccctcacagt ggggaaaaat
     3181 gaggaggatg ctaaaaagta ttttcttgat cagatcgaag tttgcagaga caaaggatgg
     3241 actgtgcagt ttaattggtt tctacatctt gttcttggca tcaaacaagg agagaaacat
     3301 tcagcc
//
