LOCUS       sequence-155010-        5231 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing WNK3, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_WNK3_p.T237M
FEATURES             Location/Qualifiers
     source          1..5231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggccactg attcagggga tccagccagc acagaagatt ctgagaaacc tgatggaatt
       61 tcatttgaaa acagagttcc ccaggtcgct gcaactttga cagtagaagc tagactaaag
      121 gagaaaaaca gtaccttctc tgcttctggg gaaactgtag aaaggaagag atttttccga
      181 aagagtgttg aaatgacgga agatgacaaa gttgccgaat catcccccaa agatgagaga
      241 attaaggctg caatgaatat tccaagagta gataagcttc cttcaaatgt gttgagaggt
      301 ggacaagaag ttaaatatga acagtgttca aagtcaacct cagaaatctc aaaagattgt
      361 ttcaaggaga aaaatgaaaa ggaaatggaa gaagaagcag aaatgaaggc tgtagctact
      421 tctcctagtg gcagattcct gaaatttgac atagaactag gaagaggagc atttaaaaca
      481 gtatataaag gactggacac tgaaacatgg gttgaggttg cttggtgtga gctgcaggac
      541 cgaaagttaa ccaaagctga gcagcaaaga ttcaaggaag aagcagagat gttgaagggt
      601 ctccagcacc ccaatatagt tcgattttat gattcctggg aatctatatt aaaaggaaag
      661 aaatgtattg tattagtgac tgaactaatg acatctggga ccttaaagat gtacttaaaa
      721 cgatttaaag tcatgaaacc aaaggtctta aggagctggt gcaggcaaat tttaaagggg
      781 ttgcagttct tgcacactag gactcctcct attattcacc gggatctgaa gtgtgacaat
      841 attttcatca cgggacccac tggatctgtg aagattggtg atctaggatt agccacctta
      901 atgcgtacct catttgctaa gagtgtcatt ggaactcctg agtttatggc tccagagatg
      961 tatgaagaac actatgatga atccgtagat gtttatgctt ttggaatgtg tatgctggaa
     1021 atggccacat cggagtatcc ttattctgag tgtcagaatg cagctcaaat ataccggaaa
     1081 gtaactagtg gcataaaacc agccagcttc aataaagtca ctgatcctga agtcaaagaa
     1141 atcattgaag gatgtattcg tcaaaacaaa tctgaaaggt tgtctatcag ggacctatta
     1201 aaccatgcat tttttgctga ggatacagga ctgagggtgg agttagcaga agaagatgat
     1261 tgctcaaatt catcccttgc tttaagactc tgggttgaag accctaaaaa attgaaaggc
     1321 aaacacaaag acaatgaagc tattgaattt agtttcaact tagaaacaga tacacctgag
     1381 gaagtagcat atgaaatggt caagtctggg ttcttccatg aaagtgattc caaagctgtt
     1441 gctaaatcca ttagagaccg ggtgacgcca ataaagaaga caagagagaa gaagcctgct
     1501 ggctgtttgg aagaacgcag ggattctcag tgcaagtcta tggggaatgt attccctcag
     1561 ccccagaata caactttacc ccttgctccc gctcagcaaa ctggggctga atgtgaagaa
     1621 actgaagttg atcaacatgt tagacaacag cttctacaaa gaaaaccaca gcagcactgc
     1681 tcctctgtta caggtgacaa tttgtctgag gcaggagctg catcagttat acattcagat
     1741 acttcaagtc agcccagtgt agcctattcc tcaaatcaaa cgatgggctc tcaaatggtt
     1801 tctaatatcc cgcaggctga agtaaatgtt ccagggcaaa tttattcatc tcagcaacta
     1861 gtaggacatt accagcaagt ttcagggtta cagaagcatt caaagctgac tcagccgcag
     1921 attttgcctt tggttcaagg tcagtccact gttttacctg tacatgtcct tggaccgaca
     1981 gttgtttcac aaccccaggt ttccccatta actgttcaga aggtcccaca gataaagcct
     2041 gtatcccaac cagttggagc tgaacaacaa gcagctcttc taaaaccaga tttagttcga
     2101 agcttgaatc aagatgtggc aactacgaag gaaaacgtca gtagccctga taacccaagt
     2161 ggaaatggca aacaggatcg gatcaaacag agaagagctt cctgtccccg accagagaag
     2221 gggactaaat ttcagcttac cgtccttcag gtatcaacct ctggagataa catggtagag
     2281 tgtcagctgg agacacacaa caacaagatg gtcaccttca agtttgatgt tgatggtgat
     2341 gcgccagagg atattgcaga ctatatggtt gaagataact ttgtgctgga aagtgagaaa
     2401 gaaaaatttg tagaagaatt gagagctatt gtaggtcaag cccaggagat ccttcatgtc
     2461 cactttgcca cagaaagagc cactggagtt gattctatta ctgtggactc caacagtagc
     2521 cagacagggt catctgaaca agtacagata aattctacat ctactcaaac cagcaatgaa
     2581 tctgctcctc agtcatcccc agttggtcgg tggcgattct gtatcaatca gacgataaga
     2641 aaccgtgaga ctcagtctcc tccttctctt cagcattcca tgtctgcggt tcctggccga
     2701 catccacttc ctagtccaaa aaacacaagt aataaggaaa tatcacggga cacattgctc
     2761 actatagaaa ataatccatg ccaccgtgca cttttcacct ccaaatcaga acacaaggat
     2821 gtggttgatg gtaagatttc tgaatgtgct agtgtagaaa ccaagcagcc agctatactt
     2881 tatcaagtgg aagataacag gcagataatg gcaccagtta ctaatagttc cagttactct
     2941 actacttcag ttcgtgcagt tccagctgaa tgtgagggac tcaccaaaca agcaagcata
     3001 ttcatacctg tgtatccatg tcaccaaact gccagtcagg ctgatgcact tatgtcccat
     3061 cctggcgaat caactcagac ttctggtaac tctcttacaa ctctggcatt tgatcaaaag
     3121 cctcaaacct tatcagtaca gcagccagct atggatgcag agtttatttc tcaagaagga
     3181 gaaactacag tgaacactga agcaagttct cctaagacag tcattcccac tcagacccct
     3241 ggccttgaac caactaccct tcaacccact actgtcctgg aatcagatgg agaaagacct
     3301 ccaaaactgg agtttgcaga caaccgaatt aaaactctgg atgaaaaatt aagaaacttg
     3361 ctctatcagg agcacagcat ctctagcatc tatcccgaga gtcagaagga tacccaaagc
     3421 atagactctc cattttcttc ctctgctgaa gataccctct cctgtccagt gacagaagtc
     3481 atagccatca gtcactgtgg aattaaagat agccctgtac aatcccctaa tttccaacag
     3541 acaggctcta agcttctgtc caatgtggct gcaagtcagc ctgctaatat atcagtgttc
     3601 aaaagggacc tgaatgtgat aacttctgta cccagcgaat tgtgtttaca tgagatgtcc
     3661 tcagatgctt cacttccagg ggatccagag gcctatcctg ctgctgtgtc aagcggtgga
     3721 gccattcatc tgcagacagg agtggaaaca gaagagatga gatcagcaat tgctcctgat
     3781 cccattcctc tgacacggga gtccacagct gatactaggg ctttgaatag atgtaaagcg
     3841 atgagtggat catttcagcg gggtcggttc caggtgatta caattcctca gcagcagtca
     3901 gcaaaaatga catcttttgg aatagaacac atatcagtgt tcagtgagac aaaccattct
     3961 agtgaagaag cctttattaa aacagcaaag tctcagttgg tagaaataga acctgccaca
     4021 caaaatccaa aaacttcgtt ttcttatgag aagttacaag ctcttcagga aacctgtaaa
     4081 gaaaataaag gagttcccaa acaaggtgac aacttcttat ctttcagcgc agcttgtgag
     4141 actgatgtat cttcagtgac cccagaaaag gaatttgaag aaacttcagc cacaggaagt
     4201 agcatgcagt ctggatctga actgttgctt aaagagagag agatattgac tgctgggaaa
     4261 cagcctagct ctgatagtga attttcagcc agtcttgctg gcagtggaaa gtcagtggca
     4321 aagactggtc cagagagtaa tcagtgctta ccacaccacg aagaacaagc ttatgctcaa
     4381 acacagagtt cactcttcta ttcgccatct tccccaatga gcagtgatga tgaatcagaa
     4441 atagaggatg aggacttgaa ggtggagctt caaagattac gagaaaaaca cattcaggag
     4501 gtggtaaatc ttcaaaccca gcagaataag gagctgcagg agctctatga acgccttcgg
     4561 tcaattaaag atagcaaaac ccaatctact gagattcctt tgccacctgc atcaccacgt
     4621 cgaccaagat ctttcaaaag caaacttcga agccgccccc agtccttgac acatgtggac
     4681 aatggcatag ttgctacaga tccactgtgt gtggagagta atgcagcatc atgccaacag
     4741 tctccagcca gtaaaaaagg gatgttcaca gatgacttac acaagctggt ggatgactgg
     4801 acaaaggaag cagtaggaaa ttctcttatt aagccaagtt taaaccaact taaacaaagt
     4861 caacacaaac tagagacaga aaactggaat aaagtatctg aaaatactcc gtctactatg
     4921 ggctacacat caacatggat ttcttctctg tcccaaatcc gtggagctgt cccaacttcc
     4981 ttgccacaag gactctcact cccttcattt cctgggccat tatcatcata tggaatgcct
     5041 cacgtttgtc agtataatgc tgtggcgggg gcggggtatc cagtacagtg ggtaggaatt
     5101 tcaggaacaa cacaacaatc tgtagtaatt cccgcccaat ctgggggacc attccagcca
     5161 gggatgaata tgcaggcatt tccaacttca tcagtgcaga atcctgccac aatccctcct
     5221 ggtcctaaat g
//
