LOCUS       sequence-155035-        5230 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing WNK3, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_WNK3_p.E417Q
FEATURES             Location/Qualifiers
     source          1..5230
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggccactg attcagggga tccagccagc acagaagatt ctgagaaacc tgatggaatt
       61 tcatttgaaa acagagttcc ccaggtcgct gcaactttga cagtagaagc tagactaaag
      121 gagaaaaaca gtaccttctc tgcttctggg gaaactgtag aaaggaagag atttttccga
      181 aagagtgttg aaatgacgga agatgacaaa gttgccgaat catcccccaa agatgagaga
      241 attaaggctg caatgaatat tccaagagta gataagcttc cttcaaatgt gttgagaggt
      301 ggacaagaag ttaaatatga acagtgttca aagtcaacct cagaaatctc aaaagattgt
      361 ttcaaggaga aaaatgaaaa ggaaatggaa gaagaagcag aaatgaaggc tgtagctact
      421 tctcctagtg gcagattcct gaaatttgac atagaactag gaagaggagc atttaaaaca
      481 gtatataaag gactggacac tgaaacatgg gttgaggttg cttggtgtga gctgcaggac
      541 cgaaagttaa ccaaagctga gcagcaaaga ttcaagggaa gaagcagaga tgttgaaggg
      601 tctccagcac cccaatatag ttcgatttta tgattcctgg gaatctatat taaaaggaaa
      661 gaaatgtatt gtattagtga ctgaactaat gacatctggg accttaaaga cgtacttaaa
      721 acgatttaaa gtcatgaaac caaaggtctt aaggagctgg tgcaggcaaa ttttaaaggg
      781 gttgcagttc ttgcacacta ggactcctcc tattattcac cgggatctga agtgtgacaa
      841 tattttcatc acgggaccca ctggatctgt gaagattggt gatctaggat tagccacctt
      901 aatgcgtacc tcatttgcta agagtgtcat tggaactcct gagtttatgg ctccagagat
      961 gtatgaagaa cactatgatg aatccgtaga tgtttatgct tttggaatgt gtatgctgga
     1021 aatggccaca tcggagtatc cttattctga gtgtcagaat gcagctcaat ataccggaag
     1081 taactagtgg cataaaacca gccagcttca ataaagtcac tgatcctgaa gtcaaagaaa
     1141 tcattgaagg atgtattcgt caaaacaaat ctgaaaggtt gtctatcagg gacctattaa
     1201 accatgcatt ttttgctgag gatacaggac tgagggtgga gttagcacaa gaagatgatt
     1261 gctcaaattc atcccttgct ttaagactct gggttgaaga ccctaaaaaa ttgaaaggca
     1321 aacacaaaga caatgaagct attgaattta gtttcaactt agaaacagat acacctgagg
     1381 aagtagcata tgaaatggtc aagtctgggt tcttccatga aagtgattcc aaagctgttg
     1441 ctaaatccat tagagaccgg gtgacgccaa taaagaagac aagagagaag aagcctgctg
     1501 gctgtttgga agaacgcagg gattctcagt gcaagtctat ggggaatgta ttccctcagc
     1561 cccagaatac aactttaccc cttgctcccg ctcagcaaac tggggctgaa tgtgaagaaa
     1621 ctgaagttga tcaacatgtt agacaacagc ttctacaaag aaaaccacag cagcactgct
     1681 cctctgttac aggtgacaat ttgtctgagg caggagctgc atcagttata cattcagata
     1741 cttcaagtca gcccagtgta gcctattcct caaatcaaac gatgggctct caaatggttt
     1801 ctaatatccc gcaggctgaa gtaaatgttc cagggcaaat ttattcatct cagcaactag
     1861 taggacatta ccagcaagtt tcagggttac agaagcattc aaagctgact cagccgcaga
     1921 ttttgccttt ggttcaaggt cagtccactg ttttacctgt acatgtcctt ggaccgacag
     1981 ttgtttcaca accccaggtt tccccattaa ctgttcagaa ggtcccacag ataaagcctg
     2041 tatcccaacc agttggagct gaacaacaag cagctcttct aaaaccagat ttagttcgaa
     2101 gcttgaatca agatgtggca actacgaagg aaaacgtcag tagccctgat aacccaagtg
     2161 gaaatggcaa acaggatcgg atcaaacaga gaagagcttc ctgtccccga ccagagaagg
     2221 ggactaaatt tcagcttacc gtccttcagg tatcaacctc tggagataac atggtagagt
     2281 gtcagctgga gacacacaac aacaagatgg tcaccttcaa gtttgatgtt gatggtgatg
     2341 cgccagagga tattgcagac tatatggttg aagataactt tgtgctggaa agtgagaaag
     2401 aaaaatttgt agaagaattg agagctattg taggtcaagc ccaggagatc cttcatgtcc
     2461 actttgccac agaaagagcc actggagttg attctattac tgtggactcc aacagtagcc
     2521 agacagggtc atctgaacaa gtacagataa attctacatc tactcaaacc agcaatgaat
     2581 ctgctcctca gtcatcccca gttggtcggt ggcgattctg tatcaatcag acgataagaa
     2641 accgtgagac tcagtctcct ccttctcttc agcattccat gtctgcggtt cctggccgac
     2701 atccacttcc tagtccaaaa aacacaagta ataaggaaat atcacgggac acattgctca
     2761 ctatagaaaa taatccatgc caccgtgcac ttttcacctc caaatcagaa cacaaggatg
     2821 tggttgatgg taagatttct gaatgtgcta gtgtagaaac caagcagcca gctatacttt
     2881 atcaagtgga agataacagg cagataatgg caccagttac taatagttcc agttactcta
     2941 ctacttcagt tcgtgcagtt ccagctgaat gtgagggact caccaaacaa gcaagcatat
     3001 tcatacctgt gtatccatgt caccaaactg ccagtcaggc tgatgcactt atgtcccatc
     3061 ctggcgaatc aactcagact tctggtaact ctcttacaac tctggcattt gatcaaaagc
     3121 ctcaaacctt atcagtacag cagccagcta tggatgcaga gtttatttct caagaaggag
     3181 aaactacagt gaacactgaa gcaagttctc ctaagacagt cattcccact cagacccctg
     3241 gccttgaacc aactaccctt caacccacta ctgtcctgga atcagatgga gaaagacctc
     3301 caaaactgga gtttgcagac aaccgaatta aaactctgga tgaaaaatta agaaacttgc
     3361 tctatcagga gcacagcatc tctagcatct atcccgagag tcagaaggat acccaaagca
     3421 tagactctcc attttcttcc tctgctgaag ataccctctc ctgtccagtg acagaagtca
     3481 tagccatcag tcactgtgga attaaagata gccctgtaca atcccctaat ttccaacaga
     3541 caggctctaa gcttctgtcc aatgtggctg caagtcagcc tgctaatata tcagtgttca
     3601 aaagggacct gaatgtgata acttctgtac ccagcgaatt gtgtttacat gagatgtcct
     3661 cagatgcttc acttccaggg gatccagagg cctatcctgc tgctgtgtca agcggtggag
     3721 ccattcatct gcagacagga gtggaaacag aagagatgag atcagcaatt gctcctgatc
     3781 ccattcctct gacacgggag tccacagctg atactagggc tttgaataga tgtaaagcga
     3841 tgagtggatc atttcagcgg ggtcggttcc aggtgattac aattcctcag cagcagtcag
     3901 caaaaatgac atcttttgga atagaacaca tatcagtgtt cagtgagaca aaccattcta
     3961 gtgaagaagc ctttattaaa acagcaaagt ctcagttggt agaaatagaa cctgccacac
     4021 aaaatccaaa aacttcgttt tcttatgaga agttacaagc tcttcaggaa acctgtaaag
     4081 aaaataaagg agttcccaaa caaggtgaca acttcttatc tttcagcgca gcttgtgaga
     4141 ctgatgtatc ttcagtgacc ccagaaaagg aatttgaaga aacttcagcc acaggaagta
     4201 gcatgcagtc tggatctgaa ctgttgctta aagagagaga gatattgact gctgggaaac
     4261 agcctagctc tgatagtgaa ttttcagcca gtcttgctgg cagtggaaag tcagtggcaa
     4321 agactggtcc agagagtaat cagtgcttac cacaccacga agaacaagct tatgctcaaa
     4381 cacagagttc actcttctat tcgccatctt ccccaatgag cagtgatgat gaatcagaaa
     4441 tagaggatga ggacttgaag gtggagcttc aaagattacg agaaaaacac attcaggagg
     4501 tggtaaatct tcaaacccag cagaataagg agctgcagga gctctatgaa cgccttcggt
     4561 caattaaaga tagcaaaacc caatctactg agattccttt gccacctgca tcaccacgtc
     4621 gaccaagatc tttcaaaagc aaacttcgaa gccgccccca gtccttgaca catgtggaca
     4681 atggcatagt tgctacagat ccactgtgtg tggagagtaa tgcagcatca tgccaacagt
     4741 ctccagccag taaaaaaggg atgttcacag atgacttaca caagctggtg gatgactgga
     4801 caaaggaagc agtaggaaat tctcttatta agccaagttt aaaccaactt aaacaaagtc
     4861 aacacaaact agagacagaa aactggaata aagtatctga aaatactccg tctactatgg
     4921 gctacacatc aacatggatt tcttctctgt cccaaatccg tggagctgtc ccaacttcct
     4981 tgccacaagg actctcactc ccttcatttc ctgggccatt atcatcatat ggaatgcctc
     5041 acgtttgtca gtataatgct gtggcggggg cggggtatcc agtacagtgg gtaggaattt
     5101 caggaacaac acaacaatct gtagtaattc ccgcccaatc tgggggacca ttccagccag
     5161 ggatgaatat gcaggcattt ccaacttcat cagtgcagaa tcctgccaca atccctcctg
     5221 gtcctaaatg
//
