LOCUS       sequence-155042-        5230 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing WNK3, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_WNK3_p.V488A
FEATURES             Location/Qualifiers
     source          1..5230
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggccactg attcagggga tccagccagc acagaagatt ctgagaaacc tgatggaatt
       61 tcatttgaaa acagagttcc ccaggtcgct gcaactttga cagtagaagc tagactaaag
      121 gagaaaaaca gtaccttctc tgcttctggg gaaactgtag aaaggaagag atttttccga
      181 aagagtgttg aaatgacgga agatgacaaa gttgccgaat catcccccaa agatgagaga
      241 attaaggctg caatgaatat tccaagagta gataagcttc cttcaaatgt gttgagaggt
      301 ggacaagaag ttaaatatga acagtgttca aagtcaacct cagaaatctc aaaagattgt
      361 ttcaaggaga aaaatgaaaa ggaaatggaa gaagaagcag aaatgaaggc tgtagctact
      421 tctcctagtg gcagattcct gaaatttgac atagaactag gaagaggagc atttaaaaca
      481 gtatataaag gactggacac tgaaacatgg gttgaggttg cttggtgtga gctgcaggac
      541 cgaaagttaa ccaaagctga gcagcaaaga ttcaaggaag aagcagagat gttgaagggt
      601 ctccagcacc ccaatatagt tcgattttat gattcctggg aatctatatt aaaaggaaag
      661 aaatgtattg tattagtgac tgaactaatg acatctggga ccttaaagac gtacttaaaa
      721 cgatttaaag tcatgaaacc aaaggtctta aggagctggt gcaggcaaat tttaaagggg
      781 ttgcagttct tgcacactag gactcctcct attattcacc gggatctgaa gtgtgacaat
      841 attttcatca cgggacccac tggatctgtg aagattggtg atctaggatt agccacctta
      901 atgcgtacct catttgctaa gagtgtcatt ggaactcctg agtttatggc tccagagatg
      961 tatgaagaac actatgatga atccgtagat gtttatgctt ttggaatgtg tatgctggaa
     1021 atggccacat cggagtatcc ttattctgag tgtcagaatg cagctcaaat ataccggaaa
     1081 gtaactagtg gcataaaacc agccagcttc aataaagtca ctgatcctga agtcaaagaa
     1141 atcattgaag gatgtattcg tcaaaacaaa tctgaaaggt tgtctatcag ggacctatta
     1201 aaccatgcat tttttgctga ggatacagga ctgagggtgg agttagcaga agaagatgat
     1261 tgctcaaatt catcccttgc tttaagactc tgggttgaag accctaaaaa attgaaaggc
     1321 aaacacaaag acaatgaagc tattgaattt agtttcaact tagaaacaga tacacctgag
     1381 gaagtagcat atgaaatggt caagtctggg ttcttccatg aaagtgattc caaagctgtt
     1441 gctaaatcca ttagagaccg ggcgacgcca ataaagaaga caagagagaa gaagcctgct
     1501 ggctgtttgg aagaacgcag ggattctcag tgcaagtcta tggggaatgt attccctcag
     1561 ccccagaata caactttacc ccttgctccc gctcagcaaa ctggggctga atgtgaagaa
     1621 actgaagttg atcaacatgt tagacaacag cttctacaaa gaaaaccaca gcagcactgc
     1681 tcctctgtta caggtgacaa tttgtctgag gcaggagctg catcagttat acattcagat
     1741 acttcaagtc agcccagtgt agcctattcc tcaaatcaaa cgatgggctc tcaaatggtt
     1801 tctaatatcc cgcaggctga agtaaatgtt ccagggcaaa tttattcatc tcagcaacta
     1861 gtaggacatt accagcaagt ttcagggtta cagaagcatt caaagctgac tcagccgcag
     1921 attttgcctt tggttcaagg tcagtccact gttttacctg tacatgtcct tggaccgaca
     1981 gttgtttcac aaccccaggt ttccccatta actgttcaga aggtcccaca gataaagcct
     2041 gtatcccaac cagttggagc tgaacaacaa gcagctcttc taaaaccaga tttagttcga
     2101 agcttgaatc aagatgtggc aactacgaag gaaaacgtca gtagccctga taacccaagt
     2161 ggaaatggca aacaggatcg gatcaaacag agaagagctt cctgtccccg accagagaag
     2221 gggactaaat ttcagcttac cgtccttcag gtatcaacct ctggagataa catggtagag
     2281 tgtcagctgg agacacacaa caacaagatg gtcaccttca gtttgatgtt gatggtgatg
     2341 cgccagagga tattgcagac tatatggttg aagataactt tgtgctggaa agtgagaaag
     2401 aaaaatttgt agaagaattg agagctattg taggtcaagc ccaggagatc cttcatgtcc
     2461 actttgccac agaaagagcc actggagttg attctattac tgtggactcc aacagtagcc
     2521 agacagggtc atctgaacaa gtacagataa attctacatc tactcaaacc agcaatgaat
     2581 ctgctcctca gtcatcccca gttggtcggt ggcgattctg tatcaatcag acgataagaa
     2641 accgtgagac tcagtctcct ccttctcttc agcattccat gtctgcggtt cctggccgac
     2701 atccacttcc tagtccaaaa aacacaagta ataaggaaat atcacgggac acattgctca
     2761 ctatagaaaa taatccatgc caccgtgcac ttttcacctc caaatcagaa cacaaggatg
     2821 tggttgatgg taagatttct gaatgtgcta gtgtagaaac caagcagcca gctatacttt
     2881 atcaagtgga agataacagg cagataatgg caccagttac taatagttcc agttactcta
     2941 ctacttcagt tcgtgcagtt ccagctgaat gtgagggact caccaaacaa gcaagcatat
     3001 tcatacctgt gtatccatgt caccaaactg ccagtcaggc tgatgcactt atgtcccatc
     3061 ctggcgaatc aactcagact tctggtaact ctcttacaac tctggcattt gatcaaaagc
     3121 ctcaaacctt atcagtacag cagccagcta tggatgcaga gtttatttct caagaaggag
     3181 aaactacagt gaacactgaa gcaagttctc ctaagacagt cattcccact cagacccctg
     3241 gccttgaacc aactaccctt caacccacta ctgtcctgga atcagatgga gaaagacctc
     3301 caaaactgga gtttgcagac aaccgaatta aaactctgga tgaaaaatta agaaacttgc
     3361 tctatcagga gcacagcatc tctagcatct atcccgagag tcagaaggat acccaaagca
     3421 tagactctcc attttcttcc tctgctgaag ataccctctc ctgtccagtg acagaagtca
     3481 tagccatcag tcactgtgga attaaagata gccctgtaca atcccctaat ttccaacaga
     3541 caggctctaa gcttctgtcc aatgtggctg caagtcagcc tgctaatata tcagtgttca
     3601 aaagggacct gaatgtgata acttctgtac ccagcgaatt gtgtttacat gagatgtcct
     3661 cagatgcttc acttccaggg gatccagagg cctatcctgc tgctgtgtca agcggtggag
     3721 ccattcatct gcagacagga gtggaaacag aagagatgag atcagcaatt gctcctgatc
     3781 ccattcctct gacacgggag tccacagctg atactagggc tttgaataga tgtaaagcga
     3841 tgagtggatc atttcagcgg ggtcggttcc aggtgattac aattcctcag cagcagtcag
     3901 caaaaatgac atcttttgga atagaacaca tatcagtgtt cagtgagaca aaccattcta
     3961 gtgaagaagc ctttattaaa acagcaaagt ctcagttggt agaaatagaa cctgccacac
     4021 aaaatccaaa aacttcgttt tcttatgaga agttacaagc tcttcaggaa acctgtaaag
     4081 aaaataaagg agttcccaaa caaggtgaca acttcttatc tttcagcgca gcttgtgaga
     4141 ctgatgtatc ttcagtgacc ccagaaaagg aatttgaaga aacttcagcc acaggaagta
     4201 gcatgcagtc tggatctgaa ctgttgctta aagagagaga gatattgact gctgggaaac
     4261 agcctagctc tgatagtgaa ttttcagcca gtcttgctgg cagtggaaag tcagtggcaa
     4321 agactggtcc agagagtaat cagtgcttac cacaccacga agaacaagct tatgctcaaa
     4381 cacagagttc actcttctat tcgccatctt ccccaatgag cagtgatgat gaatcagaaa
     4441 tagaggatga ggacttgaag gtggagcttc aaagattacg agaaaaacac attcaggagg
     4501 tggtaaatct tcaaacccag cagaataagg agctgcagga gctctatgaa cgccttcggt
     4561 caattaaaga tagcaaaacc caatctactg agattccttt gccacctgca tcaccacgtc
     4621 gaccaagatc tttcaaaagc aaacttcgaa gccgccccca gtccttgaca catgtggaca
     4681 atggcatagt tgctacagat ccactgtgtg tggagagtaa tgcagcatca tgccaacagt
     4741 ctccagccag taaaaaaggg atgttcacag atgacttaca caagctggtg gatgactgga
     4801 caaaggaagc agtaggaaat tctcttatta agccaagttt aaaccaactt aaacaaagtc
     4861 aacacaaact agagacagaa aactggaata aagtatctga aaatactccg tctactatgg
     4921 gctacacatc aacatggatt tcttctctgt cccaaatccg tggagctgtc ccaacttcct
     4981 tgccacaagg actctcactc ccttcatttc ctgggccatt atcatcatat ggaatgcctc
     5041 acgtttgtca gtataatgct gtggcggggg cggggtatcc agtacagtgg gtaggaattt
     5101 caggaacaac acaacaatct gtagtaattc ccgcccaatc tgggggacca ttccagccag
     5161 ggatgaatat gcaggcattt ccaacttcat cagtgcagaa tcctgccaca atccctcctg
     5221 gtcctaaatg
//
