LOCUS       sequence-155245-        3640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing EGFR, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_EGFR_WT
FEATURES             Location/Qualifiers
     source          1..3640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..72,73..1935,1936..2004,2005..3633)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
ORIGIN
        1 atgcgaccct ccgggacggc cggggcagcg ctcctggcgc tgctggctgc gctctgcccg
       61 gcgagtcggg ctctggagga aaagaaagtt tgccaaggca cgagtaacaa gctcacgcag
      121 ttgggcactt ttgaagatca ttttctcagc ctccagagga tgttcaataa ctgtgaggtg
      181 gtccttggga atttggaaat tacctatgtg cagaggaatt atgatctttc cttcttaaag
      241 accatccagg aggtggctgg ttatgtcctc attgccctca acacagtgga gcgaattcct
      301 ttggaaaacc tgcagatcat cagaggaaat atgtactacg aaaattccta tgccttagca
      361 gtcttatcta actatgatgc aaataaaacc ggactgaagg agctgcccat gagaaattta
      421 caggaaatcc tgcatggcgc cgtgcggttc agcaacaacc ctgccctgtg caacgtggag
      481 agcatccagt ggcgggacat agtcagcagt gactttctca gcaacatgtc gatggacttc
      541 cagaaccacc tgggcagctg ccaaaagtgt gatccaagct gtcccaatgg gagctgctgg
      601 ggtgcaggag aggagaactg ccagaaactg accaaaatca tctgtgccca gcagtgctcc
      661 gggcgctgcc gtggcaagtc ccccagtgac tgctgccaca accagtgtgc tgcaggctgc
      721 acaggccccc gggagagcga ctgcctggtc tgccgcaaat tccgagacga agccacgtgc
      781 aaggacacct gccccccact catgctctac aaccccacca cgtaccagat ggatgtgaac
      841 cccgagggca aatacagctt tggtgccacc tgcgtgaaga agtgtccccg taattatgtg
      901 gtgacagatc acggctcgtg cgtccgagcc tgtggggccg acagctatga gatggaggaa
      961 gacggcgtcc gcaagtgtaa gaagtgcgaa gggccttgcc gcaaagtgtg taacggaata
     1021 ggtattggtg aatttaaaga ctcactctcc ataaatgcta cgaatattaa acacttcaaa
     1081 aactgcacct ccatcagtgg cgatctccac atcctgccgg tggcatttag gggtgactcc
     1141 ttcacacata ctcctcctct ggatccacag gaactggata ttctgaaaac cgtaaaggaa
     1201 atcacagggt ttttgctgat tcaggcttgg cctgaaaaca ggacggacct ccatgccttt
     1261 gagaacctag aaatcatacg cggcaggacc aagcaacatg gtcagttttc tcttgcagtc
     1321 gtcagcctga acataacatc cttgggatta cgctccctca aggagataag tgatggagat
     1381 gtgataattt caggaaacaa aaatttgtgc tatgcaaata caataaactg gaaaaaactg
     1441 tttgggacct ccggtcagaa aaccaaaatt ataagcaaca gaggtgaaaa cagctgcaag
     1501 gccacaggcc aggtctgcca tgccttgtgc tcccccgagg gctgctgggg cccggagccc
     1561 agggactgcg tctcttgccg gaatgtcagc cgaggcaggg aatgcgtgga caagtgcaac
     1621 cttctggagg gtgagccaag ggagtttgtg gagaactctg agtgcataca gtgccaccca
     1681 gagtgcctgc ctcaggccat gaacatcacc tgcacaggac ggggaccaga caactgtatc
     1741 cagtgtgccc actacattga cggcccccac tgcgtcaaga cctgcccggc aggagtcatg
     1801 ggagaaaaca acaccctggt ctggaagtac gcagacgccg gccatgtgtg ccacctgtgc
     1861 catccaaact gcacctacgg atgcactggg ccaggtcttg aaggctgtcc aacgaatggg
     1921 cctaagatcc cgtccatcgc cactgggatg gtgggggccc tcctcttgct gctggtggtg
     1981 gccctgggga tcggcctctt catgcgaagg cgccacatcg ttcggaagcg cacgctgcgg
     2041 aggctgctgc aggagaggga gcttgtggag cctcttacac ccagtggaga agctcccaac
     2101 caagctctct tgaggatctt gaaggaaact gaattcaaaa agatcaaagt gctgggctcc
     2161 ggtgcgttcg gcacggtgta taagggactc tggatcccag aaggtgagaa agttaaaatt
     2221 cccgtcgcta tcaaggaatt aagagaagca acatctccga aagccaacaa ggaaatcctc
     2281 gatgaagcct acgtgatggc cagcgtggac aacccccacg tgtgccgcct gctgggcatc
     2341 tgcctcacct ccaccgtgca actcatcacg cagctcatgc ccttcggctg cctcctggac
     2401 tatgtccggg aacacaaaga caatattggc tcccagtacc tgctcaactg gtgtgtgcag
     2461 atcgcaaagg gcatgaacta cttggaggac cgtcgcttgg tgcaccgcga cctggcagcc
     2521 aggaacgtac tggtgaaaac accgcagcat gtcaagatca cagattttgg gctggccaaa
     2581 ctgctgggtg cggaagagaa agaataccat gcagaaggag gcaaagtgcc tatcaagtgg
     2641 atggcattgg aatcaatttt acacagaatc tatacccacc agagtgatgt ctggagctac
     2701 ggggtgaccg tttgggagtt gatgaccttt ggatccaagc catatgacgg aatccctgcc
     2761 agcgagatct cctccatcct ggagaaagga gaacgcctcc ctcagccacc catatgtacc
     2821 atcgatgtct acatgatcat ggtcaagtgc tggatgatag acgcagatag tcgcccaaag
     2881 ttccgtgagt tgatcatcga attctccaaa atggcccgag acccccagcg ctaccttgtc
     2941 attcaggggg atgaaagaat gcatttgcca agtcctacag actccaactt ctaccgtgcc
     3001 ctgatggatg aagaagacat ggacgacgtg gtggatgccg acgagtacct catcccacag
     3061 cagggcttct tcagcagccc ctccacgtca cggactcccc tcctgagctc tctgagtgca
     3121 accagcaaca attccaccgt ggcttgcatt gatagaaatg ggctgcaaag ctgtcccatc
     3181 aaggaagaca gcttcttgca gcgatacagc tcagacccca caggcgcctt gactgaggac
     3241 agcatagacg acaccttcct cccagtgcct gaatacataa accagtccgt tcccaaaagg
     3301 cccgctggct ctgtgcagaa tcctgtctat cacaatcagc ctctgaaccc cgcgcccagc
     3361 agagacccac actaccagga cccccacagc actgcagtgg gcaaccccga gtatctcaac
     3421 actgtccagc ccacctgtgt caacagcaca ttcgacagcc ctgcccactg ggcccagaaa
     3481 ggcagccacc aaattagcct ggacaaccct gactaccagc aggacttctt tcccaaggaa
     3541 gccaagccaa atggcatctt taagggctcc acagctgaaa atgcagaata cctaagggtc
     3601 gcgccacaaa gcagtgaatt tattggagca tgattgccaa
//
