LOCUS       sequence-155341-        1284 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A ZipGFP-based Caspase-3 protease reporter T2A mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   To TL, Schepis A, Ruiz-Gonzalez R, Zhang Q, Yu D, Dong Z, Coughlin 
            SR, Shu X
  TITLE     Rational Design of a GFP-Based Fluorogenic Caspase Reporter for 
            Imaging Apoptosis In Vivo.
  JOURNAL   Cell Chem Biol. 2016 Jul 21;23(7):875-82. doi: 
            10.1016/j.chembiol.2016.06.007.
  PUBMED    27447051
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2016.06"; journalName: "Cell Chem Biol"; date: 
            "2016-07-21- 21"; volume: "23"; issue: "7"; pages: "875-82"
COMMENT     Sequence Label: ZipGFP-Casp3
FEATURES             Location/Qualifiers
     source          1..1284
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             118..165
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             289..342
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      349..358
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             469..1107
                     /codon_start=1
                     /product="fragment of superfolder GFP consisting of the 
                     first 10 beta-strands"
                     /label=GFP(1-10)
                     /note="associates with GFP11, the 11th beta-strand, to 
                     reconstitute fluorescent GFP (Cabantous et al., 2005; 
                     Kamiyama et al., 2016)"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGK
                     YKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEK"
ORIGIN
        1 atgggcggca gcaaggtgtc tgcacttaaa gaaaaggttt ccgcactcaa ggagaaagtt
       61 tcagctttga aagagaaggt cagtgccctt aaggaaaaag tgagcgcgct gaaagaacgg
      121 gaccacatgg tgctgcacga gtacgtgaac gccgccggca tcacaggtga tgaagttgat
      181 ggtgaagtga gcgcgctgga aaaagaggtc tccgctcttg agaaggaagt ttctgccttg
      241 gagaaagagg tgagcgccct ggagaaggaa gtctctgcgc ttgaaaaaga gggcagagga
      301 agtctgctaa catgcggtga cgtcgaggag aatcctggcc caaagcttgc caccatggaa
      361 gtgagcgcgc tggaaaaaga ggtctccgct cttgagaagg aagtttctgc cttggagaaa
      421 gaggtgagcg ccctggagaa ggaagtctct gcgcttgaaa aaggatcctc caaaggagaa
      481 gaactgttta ccggtgttgt gccaattttg gttgaactcg atggtgatgt caacggacat
      541 aagttctcag tgagaggcga aggagaaggt gacgccacca ttggaaaatt gactcttaaa
      601 ttcatctgta ctactggtaa acttcctgta ccatggccga ctctcgtaac aacgcttacg
      661 tacggagttc agtgcttttc gagataccca gaccatatga aaagacatga cttttttaag
      721 tcggctatgc ctgaaggtta cgtgcaagaa agaacaattt cgttcaaaga tgatggaaaa
      781 tataaaacta gagcagttgt taaatttgaa ggagatactt tggttaaccg cattgaactg
      841 aaaggaacag attttaaaga agatggtaat attcttggac acaaactcga atacaatttt
      901 aatagtcata acgtatacat cactgctgat aagcaaaaga acggaattaa agcgaatttc
      961 acagtacgcc ataatgtaga agatggcagt gttcaacttg ccgaccatta ccaacaaaac
     1021 acccctattg gagacggtcc ggtacttctt cctgataatc actacctctc aacacaaaca
     1081 gtcctgagca aagatccaaa tgaaaaaggt gatgaagttg atggtgaatt cggcggcagc
     1141 aaggtgtctg cacttaaaga aaaggtttcc gcactcaagg agaaagtttc agctttgaaa
     1201 gagaaggtca gtgcccttaa ggaaaaagtg agcgcgctga aagaaggcgg ccgctctaga
     1261 actatagtga gtcgtattac gtag
//
