LOCUS       sequence-155347-        5990 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Zhao B, Ye X, Yu J, Li L, Li W, Li S, Yu J, Lin JD, Wang CY, 
            Chinnaiyan AM, Lai ZC, Guan KL
  TITLE     TEAD mediates YAP-dependent gene induction and growth control.
  JOURNAL   Genes Dev. 2008 Jul 15. 22(14):1962-71.
  PUBMED    18579750
REFERENCE   2  (bases 1 to 5990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Jul 15. 22(14):1962-71."
COMMENT     Sequence Label: pCMX-Gal4-TEAD4
FEATURES             Location/Qualifiers
     source          1..5990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(30..52)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     152..171
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        316..695
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        696..899
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     850..870
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     944..963
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        944..962
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             995..1435
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    1451..1475
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2559..2583)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          2723..2788
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2918..2938
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3210..3344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3247..3266)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3301..3320
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3802..3936
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4001..4018)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4172..4760)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4252..4271)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4931..5722,5723..5791))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5554..5573
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5792..5896)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5964..5982
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
       61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
      181 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
      241 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
      301 ttacgccagc gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
      361 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      421 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      481 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      541 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      601 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      661 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
      721 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
      781 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
      841 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
      901 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      961 gggagaccca agcttgaagc aagcctcctg aaagatgaag ctactgtctt ctatcgaaca
     1021 agcatgcgat atttgccgac ttaaaaagct caagtgctcc aaagaaaaac cgaagtgcgc
     1081 caagtgtctg aagaacaact gggagtgtcg ctactctccc aaaaccaaaa ggtctccgct
     1141 gactagggca catctgacag aagtggaatc aaggctagaa agactggaac agctatttct
     1201 actgattttt cctcgagaag accttgacat gattttgaaa atggattctt tacaggatat
     1261 aaaagcattg ttaacaggat tatttgtaca agataatgtg aataaagatg ccgtcacaga
     1321 tagattggct tcagtggaga ctgatatgcc tctaacattg agacagcata gaataagtgc
     1381 gacatcatca tcggaagaga gtagtaacaa aggtcaaaga cagttgactg tatcgccgga
     1441 attcccatca acaagtttgt acaaaaaagt tggcatgtat ggtcggaacg agctgattgc
     1501 ccgctacatc aagctccgga cagggaagac ccgcaccagg aagcaggtct ccagccacat
     1561 ccaggtgctg gctcgtcgca aagctcgcga gatccaggcc aagctaaagg accaggcagc
     1621 taaggacaag gccctgcaga gcatggctgc catgtcgtct gcacagatca tctccgccac
     1681 ggccttccac agtagcatgg ccctcgcccg gggccccggc cgcccagcag tctcagggtt
     1741 ttggcaagga gctttgccag gccaagccgg aacgtcccat gatgtgaagc ctttctctca
     1801 gcaaacctat gctgtccagc ctccgctgcc tctgccaggg tttgagtctc ctgcagggcc
     1861 cgccccatcg ccctctgcgc ccccggcacc cccatggcag ggccgcagcg tggccagctc
     1921 caagctctgg atgttggagt tctctgcctt cctggagcag cagcaggacc cggacacgta
     1981 caacaagcac ctgttcgtgc acattggcca gtccagccca agctacagcg acccctacct
     2041 cgaagccgtg gacatccgcc aaatctatga caaattcccg gagaaaaagg gtggactcaa
     2101 ggatctcttc gaacggggac cctccaatgc cttttttctt gtgaagttct gggcagacct
     2161 caacaccaac atcgaggatg aaggcagctc cttctatggg gtctccagcc agtatgagag
     2221 ccccgagaac atgatcatca cctgctccac gaaggtctgc tctttcggca agcaggtggt
     2281 ggagaaagtt gagacagagt atgctcgcta tgagaatgga cactactctt accgcatcca
     2341 ccggtccccg ctctgtgagt acatgatcaa cttcatccac aagctcaagc acctccctga
     2401 gaagtacatg atgaacagcg tgctggagaa cttcaccatc ctgcaggtgg tcaccaacag
     2461 agacacacag gagaccttgc tgtgcattgc ctatgtcttt gaggtgtcag ccagtgagca
     2521 cggggctcag caccacatct acaggctggt gaaagaatgc ccaactttct tgtacaaagt
     2581 ggttgatggg gatccgtcga cggtaccaga tatcaggatc ctggccagct agctaggtag
     2641 ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     2701 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     2761 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     2821 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     2881 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     2941 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     3001 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     3061 gaaaattatg gaaaaatatt tgatgtatag tgccttgact agagatcata atcagccata
     3121 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     3181 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     3241 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     3301 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcagcttc agaagatggc
     3361 ggagggcctc caacacagta attttcctcc cgactcttaa aatagaaaat gtcaagtcag
     3421 ttaagcagga agtgactaac tgaccgcagc tggccgtgcg acatcctctt ttaattagtt
     3481 gctaggcaac tgccctccag agggcagtgt ggttttgcaa gaggaagcaa aaagcctctc
     3541 cacccaggcc tagaatgttt ccacccaatc attactatga caacagctgt tttttttagt
     3601 attaagcaga ggccgggggc ccctggcctc cgcttactct ggagaaaaag aagagaggca
     3661 ttgtagaggc ttccagaggc aacttgtcaa aacaggactg cttctatttc tgtcacactg
     3721 tctggccctg tcacaaggtc cagcacctcc ataccccctt taataagcag tttgggaacg
     3781 ggtgcgggtc ttactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     3841 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     3901 ctattccaga agtagtgagg aggctttttt ggaggctgca ttaatgaatc ggccaacgcg
     3961 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     4021 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4081 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4141 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4201 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4261 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4321 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4381 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4441 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4501 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4561 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4621 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4681 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4741 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4801 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4861 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4921 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4981 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5041 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5101 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5161 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5221 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5281 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5341 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5401 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5461 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5521 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     5581 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     5641 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5701 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5761 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     5821 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     5881 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     5941 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
//
