LOCUS       sequence-155351-         595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAVS1 sgRNA expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pXAT2
FEATURES             Location/Qualifiers
     source          1..595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..31
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taagatggcc ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa
       61 gaagtacagc atcggcctgg acatcggcac caactctgtg ggctgggccg tgatcaccga
      121 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
      181 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
      241 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
      301 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
      361 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
      421 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
      481 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
      541 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtg
//
