LOCUS       sequence-155361-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAVS1 TALEN expression vector (Left).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pTALdNC-AAVS1_T1
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             142..162
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             163..189
                     /codon_start=1
                     /product="epitope tag from the baculovirus Autographa 
                     californica GP64 envelope fusion protein"
                     /label=AcV5 tag
                     /translation="SWKDASGWS"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgataa gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg
      121 atcaccatgg cttcctcccc tccaaagaaa aagagaaagg ttagttggaa ggacgcaagt
      181 ggttggtcta gaatgcatgc ggccccgcga cggcgtgctg cgcaaccctc cgacgcttcg
      241 ccggccgcgc aggtggatct acgcacgctc ggctacagtc agcagcagca agagaagatc
      301 aaaccgaagg tgcgttcgac agtggcgcag caccacgagg cactggtggg ccatgggttt
      361 acacacgcgc acatcgttgc gctcagccaa cacccggcag cgttagggac cgtcgctgtc
      421 acgtatcagc acataatcac ggcgttgcca gaggcgacac acgaagacat cgttggcgtc
      481 ggcaaacagt ggtccggcgc acgcgccctg gaggccttgc tcacggatgc gggggagttg
      541 agaggtccgc cgttacagtt ggacacaggc caacttgtga agattgcaaa acgtggcggc
      601 gtgaccgcaa tggaggcagt gcatgcatcg cgcaatgcac tgacgggtgc ccccctgaac
      661 ctgaccccgg accaagtggt ggctatcgcc agcaacattg gcggcaagca agcgctcgaa
      721 acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg
      781 gtggctatcg ccagcaacgg tggcggca
//
