LOCUS       sequence-155367-         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAVS1 TALEN expression vector (Right).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Oceguera-Yanez F, Kim SI, Matsumoto T, Tan GW, Xiang L, Hatani T, 
            Kondo T, Ikeya M, Yoshida Y, Inoue H, Woltjen K
  TITLE     Engineering the AAVS1 locus for consistent and scalable transgene 
            expression in human iPSCs and their differentiated derivatives.
  JOURNAL   Methods. 2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012.
  PUBMED    26707206
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2015 Dec 18. pii: S1046-2023(15)30181-X. doi: 
            10.1016/j.ymeth.2015.12.012."
COMMENT     Sequence Label: pTALdNC-AAVS1_T2
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    88..187
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(217..804)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
       61 acacgggcca gagctgcagc tggatggcaa ataatgattt tattttgact gatagtgacc
      121 tgttcgttgc aacaaattga taagcaatgc tttcttataa tgccaacttt gtacaagaaa
      181 gctgggtcta gatatctcga gtgcggcggc ctattaaaag tttatctcac cgttattaaa
      241 tttccttctc acttcctcaa gtgtcaatgt accagcttta atcatttctc caccaatcaa
      301 aagctcttct acactaagaa cagctccatt acaattagtg atatgattca atcttgtaag
      361 ctgagctttg tagtttcctt tgaaatgacc actcacaaac aagaacttaa attctgttac
      421 tgaagatgga tagactttcc accattcatt agggttgata tgcttgtttc ttgtttgatt
      481 ctcttcgaca tatctttgca tttcatctgc ttgaccaatt ggaagattat aacctcctga
      541 atatgcctta gtatcaacga tcacaccgta atcaatagga gatccgacag tataaattgc
      601 tccgtctggt ttccttgatc cacccaaatg tttaccacga taaccataaa ccttcataaa
      661 gaactccatc accttcattt caaggattct atcctgagtt gaatttcttg cgatttcaat
      721 caattcaata tattcatgtg gcacatattt caatttatgt ctaagttcag atttcttctc
      781 ttccaattca gatttcacta gctgg
//
