LOCUS       sequence-155370-         641 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  piggyBac transposon for dox-inducible expression of the OKMS (Oct4, 
            Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette 
            (with mCherry).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K
  TITLE     KLF4 N-terminal variance modulates induced reprogramming to 
            pluripotency.
  JOURNAL   Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 
            10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.02.004"; journalName: "Stem Cell Reports"; 
            date: "2015-04-14- 14"; volume: "4"; issue: "4"; pages: "727-43"
COMMENT     Sequence Label: PB-TAC-OKMS
FEATURES             Location/Qualifiers
     source          1..641
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(80..145)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
ORIGIN
        1 ccgaagcttg ctgcgggccc ggcggcttca gctccgtctc catcatgtta tacatggtga
       61 agggcgaatt gttcatgagt ggacctggat tgctttctac atccccagcc agtttgagta
      121 aatcaaagtt aagagtttgt ttgacaggag cgacaatttt gagatcccct gcaccagagt
      181 ttcgaagctg ttcgagtttg tgtttcaact gttctcgtcg tttcctcaat aagtcctttt
      241 cagaggtgag cttgtgctcg tctgcttgaa tggacaggat gtaggcggtg gcttttttga
      301 ggatcactac cttgggggcc ttttcgttgt tttccaattc agggatctgg tcacgcaggg
      361 caaaaaagct gcgcttcagc tcgttcctcc tctgacgttc caagacgttg tgtgtccgcc
      421 tcttgtcgtt ttcctccgtg tctgaggacc tggggctgga gcacttgcgg ttgttgctga
      481 tctgcttcag gaccctgcca ctgtccaact tggccctctt ggcagctgga tagtccttcc
      541 ttgtggaggg gggtgcggcg tagttgtgct ggtgagtgga gacgtggcac ctcttgagga
      601 ccagtgggct gtgcggaggt ttgctgtggc ctcgggatgg a
//
