LOCUS       sequence-155384-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Updated TALEN vector; compatible with the Golden Gate TALEN assembly
            protocol. More stable backbone with reduced recombination compared 
            to pT3Ts-goldyTALEN due to the removal of a lacZ sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Welker JM, Wierson WA, Wang Y, Poshusta TL, McNulty MS, Tisdale EE, 
            Solin SL, Ekker SC, Clark KJ, McGrail M, Essner JJ
  TITLE     GoldyTALEN Vectors with Improved Efficiency for Golden Gate TALEN 
            Assembly.
  JOURNAL   Hum Gene Ther. 2016 Jun;27(6):423-4. doi: 10.1089/hum.2016.012. Epub
            2016 May 6.
  PUBMED    27094430
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/hum.2016.012"; journalName: "Hum Gene Ther"; date: 
            "2016-06"; volume: "27"; issue: "6"; pages: "423-4"
COMMENT     Sequence Label: pKT3Ts-goldyTALEN
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             90..677
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     3'UTR           716..841
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
ORIGIN
        1 aaaagggatt gccgcacgcg ccggaattga tcagaagagt caatcgccgt attggcgaac
       61 gcacgtccca tcgcgttgcc tctagatccc agctagtgaa atctgaattg gaagagaaga
      121 aatctgaact tagacataaa ttgaaatatg tgccacatga atatattgaa ttgattgaaa
      181 tcgcaagaaa ttcaactcag gatagaatcc ttgaaatgaa ggtgatggag ttctttatga
      241 aggtttatgg ttatcgtggt aaacatttgg gtggatcaag gaaaccagac ggagcaattt
      301 atactgtcgg atctcctatt gattacggtg tgatcgttga tactaaggca tattcaggag
      361 gttataatct tccaattggt caagcagatg aaatgcaaag atatgtcgaa gagaatcaaa
      421 caagaaacaa gcatatcaac cctaatgaat ggtggaaagt ctatccatct tcagtaacag
      481 aatttaagtt cttgtttgtg agtggtcatt tcaaaggaaa ctacaaagct cagcttacaa
      541 gattgaatca tatcactaat tgtaatggag ctgttcttag tgtagaagag cttttgattg
      601 gtggagaaat gattaaagct ggtacattga cacttgagga agtgagaagg aaatttaata
      661 acggtgagat aaacttttaa taggctagtg actgactagg atctggttac cactaaacca
      721 gcctcaagaa cacccgaatg gagtctctaa gctacataat accaacttac actttacaaa
      781 atgttgtccc ccaaaatgta gccattcgta tctgctccta ataaaaagaa agtttcttca
      841 cattctaaaa a
//
