LOCUS       sequence-155391-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas9 and chloramphenicol acetyltransferase (CAT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, 
            Saeij JP, Carruthers VB, Niles JC, Lourido S
  TITLE     A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential 
            Apicomplexan Genes.
  JOURNAL   Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 
            10.1016/j.cell.2016.08.019. Epub 2016 Sep 2.
  PUBMED    27594426
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.08.019"; journalName: "Cell"; date: "2016-09-8-
            8"; volume: "166"; issue: "6"; pages: "1423-1435.e12"
COMMENT     Sequence Label: pCas9-CAT
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aacaactgat agctgtcttg tgtattgacc catgtggcgc gtgtgtcgac ggcgatcaac
       61 atcagccagg tccgccacac atccgttcag agattcggac ccagtcataa actttcgctg
      121 cttgctgcga aacactccta tttagagtcg gccggttttt gtggatgtga cacctgcaag
      181 ccacaacgga acaactcagt ttctttataa tggggcacat gctgcacgaa gtgtgtttcc
      241 tttgtcgatt tgagaagtga gcacaacggt gattaattaa gccccgccct gccactcatc
      301 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
      361 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
      421 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
      481 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
      541 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
      601 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
      661 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacggaa
      721 ttccggatga gcattcatca ggcgggcaag aatgtgaata aatgccggat aaaacttgtg
      781 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
      841 ggtacattga gcaactgact g
//
