LOCUS       sequence-155392-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas9 and chloramphenicol acetyltransferase (CAT).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, 
            Saeij JP, Carruthers VB, Niles JC, Lourido S
  TITLE     A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential 
            Apicomplexan Genes.
  JOURNAL   Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 
            10.1016/j.cell.2016.08.019. Epub 2016 Sep 2.
  PUBMED    27594426
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.08.019"; journalName: "Cell"; date: "2016-09-8-
            8"; volume: "166"; issue: "6"; pages: "1423-1435.e12"
COMMENT     Sequence Label: pCas9-CAT
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..50)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tactttttct tttttgcctg gccggccttt ttcgtggccg ccggcctttt gtcgcctccc
       61 agctgagaca ggtcgatccg tgtctcgtac aggccggtga tgctctggtg gatcagggtg
      121 gcgtccagca cctctttggt gctggtgtac ctcttccggt cgatggtggt gtcaaagtac
      181 ttgaaggcgg caggggctcc cagattggtc agggtaaaca ggtggatgat attctcggcc
      241 tgctctctga tgggcttatc ccggtgcttg ttgtaggcgg acagcacttt gtccagatta
      301 gcgtcggcca ggatcactct cttggagaac tcgctgatct gctcgatgat ctcgtccagg
      361 tagtgcttgt gctgttccac aaacagctgt ttctgctcat tatcctcggg ggagcccttc
      421 agcttctcat agtggctggc caggtacagg aagttcacat atttggaggg cagggccagt
      481 tcgtttccct tctgcagttc gccggcagag gccagcattc tcttccggcc gttttccagc
      541 tcgaacaggg agtacttagg cagcttgatg atcaggtcct ttttcacttc tttgtagccc
      601 ttggcttcca gaaagtcgat gggattcttc tcgaagctgc ttctttccat gatggtgatc
      661 cccagcagct ctttcacact cttcagtttc ttggacttgc ccttttccac tttggccacc
      721 accagcacag aataggccac ggtggggctg tcgaagccgc cgtacttctt agggtcccag
      781 tccttctttc tggcgatcag cttatcgctg ttcctcttgg gcaggataga ctctttgctg
      841 aagccgcctg tctgcacctc ggtctttttc acgatattca cttggggcat gctcagcact
      901 ttccgcacgg tggcaaaatc cc
//
