LOCUS       sequence-155395-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in 
            Toxoplasma gondii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, 
            Saeij JP, Carruthers VB, Niles JC, Lourido S
  TITLE     A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential 
            Apicomplexan Genes.
  JOURNAL   Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 
            10.1016/j.cell.2016.08.019. Epub 2016 Sep 2.
  PUBMED    27594426
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.08.019"; journalName: "Cell"; date: "2016-09-8-
            8"; volume: "166"; issue: "6"; pages: "1423-1435.e12"
COMMENT     Sequence Label: pU6-Decoy
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(311..331)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(338..403)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 agctgtcgtc caccttggcc atctcgttgc tgaagatctc ttgcagatag cagatccggt
       61 tcttccgtct ggtgtatctt cttctggcgg ttctcttcag ccgggtggcc tcggctgttt
      121 cgccgctgtc gaacagcagg gctccgatca ggttcttctt gatgctgtgc cggtcggtgt
      181 tgcccagcac cttgaatttc ttgctgggca ccttgtactc gtcggtgatc acggcccagc
      241 ccacagagtt ggtgccgatg tccaggccga tgctgtactt cttgtcggct gctgggactc
      301 cgtggatacc gaccttccgc ttcttctttg gggccatctt atcgtcatcg tctttgtaat
      361 caatatcatg atccttgtag tctccgtcgt ggtccttata gtccatggtg tcgaaaaagg
      421 gaattcaaga aaaaatgcca acgagtagtt ttccgcaaga acttcacaca aggtgattgt
      481 gtaacaccgt ttggtggatg tcttctgcgc gggttgcgtc gtcagggacg gcgaacgagt
      541 gcttgttctc tacgagactc gcaaagagaa ggaaagggcg aaattcaaga gagaatccaa
      601 caaggaggac tctctgctaa tattcgccgc ctttaacaca tctaaaagtt cacagagaac
      661 aaacaaaatg tttctgacac atccccttcg tgcgcgcgca tctccaccgg tccaaccagg
      721 atcaccaacc gcacccaatg caggcaccac ccctgttctg cgatgagcgc aagccgtcct
      781 gagttacgcc ttcggcatct gactgcccac cgcgagccgg cctgctccgc aacgtcatcc
      841 cgctgagacg atgattcctg atcactccgc tgatgcagag aatttcacga acgc
//
