LOCUS       sequence-155396-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows for expression of an sgRNA in Toxoplasma gondii and confers 
            resistance to pyrimethamine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, 
            Saeij JP, Carruthers VB, Niles JC, Lourido S
  TITLE     A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential 
            Apicomplexan Genes.
  JOURNAL   Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 
            10.1016/j.cell.2016.08.019. Epub 2016 Sep 2.
  PUBMED    27594426
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.08.019"; journalName: "Cell"; date: "2016-09-8-
            8"; volume: "166"; issue: "6"; pages: "1423-1435.e12"
COMMENT     Sequence Label: pU6-DHFR
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        626..701
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
       61 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgggatgaga
      121 caaagtgcgc gagttgaaat cgtcgtgggg acgatttcac cgcggccaca tgttggagac
      181 actgagggca cacgggaaac gcgaaagatt tcaaattaac gtacccaaac gcgaaagctt
      241 gcgcagcata cactcgaagc gaacatcccg aaccatcgag aggcggagag cgataagtct
      301 ttcacgctgc gaagtgttgc gacggctgcg ccgctgcact gtgaattggg cgccaatatt
      361 gcatcctagg cctgacgcgc ctcctgcaga acgcgagaca ctgggatatg tagagccaag
      421 ggggaaacct tcgaactctc gaatgtcttc tctgacaaga atcatatttc catcagttct
      481 gtcagatttt caaatggcga cctgcagagg cctgcttcct ccctgtgcgc tcttcgaagg
      541 ggctttctgt cgcgcagggt cacctcgtcc ccgaaggggg tgtttgcctt ctggtaaatg
      601 gggatgtcaa gttgagaccg gtctcgtttt agagctagaa atagcaagtt aaaataaggc
      661 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttttt ctttttctct
      721 agaggtacca tgcatctagc atgtcattcg attttcaccc cccgcgtagt tcctgtgtgt
      781 cattcgttgt cgagacaact ctgtcccgcc ccggtgctgt tccatatgcg tgactttccc
      841 gcaatttttt cagactttca ggaaagacag gctccggaac gatctcgtcc atgac
//
