LOCUS       sequence-155400-         477 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  piggyBac transposon for dox-inducible expression of the MKOS 
            polycistronic reprogramming cassette (with mCherry).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Kim SI, Oceguera-Yanez F, Hirohata R, Linker S, Okita K, Yamada Y, 
            Yamamoto T, Yamanaka S, Woltjen K
  TITLE     KLF4 N-terminal variance modulates induced reprogramming to 
            pluripotency.
  JOURNAL   Stem Cell Reports. 2015 Apr 14;4(4):727-43. doi: 
            10.1016/j.stemcr.2015.02.004. Epub 2015 Mar 12.
  PUBMED    25772473
REFERENCE   2  (bases 1 to 477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2015.02.004"; journalName: "Stem Cell Reports"; 
            date: "2015-04-14- 14"; volume: "4"; issue: "4"; pages: "727-43"
COMMENT     Sequence Label: PB-TAC-MKOS
FEATURES             Location/Qualifiers
     source          1..477
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(221..240)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     450..467
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 actcccttga tgatggccat gttatcctcc tcgcccttgc tcaccatggt tgtggccata
       61 ttatcatcgt gtttttcaaa ggaaaaccac gtccccgtgg ttcggggggc ctagacgttt
      121 ttttaacctc gactaaacac atgtaaagca tgtgcaccga ggccccagat cagatcccat
      181 acaatggggt accttctggg catccttcag ccccttgttg aatacgcttg aggagagcca
      241 tttgactctt tccacaacta tccaactcac aacgtggcac tggggttgtg ccgcctttgc
      301 aggtgtatct tatacacgtg gcttttggcc gcagaggcac ctgtcgccag gtggggggtt
      361 ccgctgcctg caaagggtcg ctacagacgt tgtttgtctt caagaagctt ccagaggaac
      421 tgcttccttc acgacattca acagaccttg cattcctttg gcgagagggg aaagacc
//
