LOCUS       sequence-155405-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sucrose counterselection (sacB) vector for unmarked allelic 
            exchange..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Puri AW, Owen S, Chu F, Chavkin T, Beck DA, Kalyuzhnaya MG, Lidstrom
            ME
  TITLE     Genetic tools for the industrially promising methanotroph 
            Methylomicrobium buryatense.
  JOURNAL   Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14.
  PUBMED    25548049
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14."
COMMENT     Sequence Label: pCM433kanT
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga
       61 tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta
      121 cgctgacttg acgggactcg agattatagc tcctgcccta tgggattcac ctttatgttg
      181 ataagaaata aaagaaaatg ccaataggat atcggcattt tcttttgcgt ttttatttgt
      241 taactgttaa ttgtccttgt tcaaggatgc tgtctttgac aacagatgtt ttcttgcctt
      301 tgatgttcag caggaagctt ggcgcaaacg ttgattgttt gtctgcgtag aatcctctgt
      361 ttgtcatata gcttgtaatc acgacattgt ttcctttcgc ttgaggtaca gcgaagtgtg
      421 agtaagtaaa ggttacatcg ttaggatcaa gatccatttt taacacaagg ccagttttgt
      481 tcagcggctt gtatgggcca gttaaagaat tagaaacata accaagcatg taaatatcgt
      541 tagacgtaat gccgtcaatc gtcatttttg atccgcggga gtcagtgaac aggtaccatt
      601 tgccgttcat tttaaagacg ttcgcgcgtt caatttcatc tgttactgtg ttagatgcaa
      661 tcagcggttt catcactttt ttcagtgtgt aatcatcgtt tagctcaatc ataccgagag
      721 cgccgtttgc taactcagcc gtgcgttttt tatcgctttg cagaagtttt tgactttctt
      781 gacggaagaa tgatgtgctt ttgccatagt atgctttgtt aaataaagat tcttcgcctt
      841 ggtagccatc ttcagttcca gtgtttgctt caaatactaa gtatttgtgg cctttatctt
      901 ctacgtagtg aggatctctc agcgtatggt tgtcgcctga gctgtagttg ccttcatcga
      961 tgaactgctg tacattttga tacgtttttc cgtcac
//
