LOCUS       sequence-155406-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis
            agglutinin).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Whitley MJ, Furey W, Kollipara S, Gronenborn AM
  TITLE     Burkholderia oklahomensis agglutinin is a canonical two-domain 
            OAA-family lectin: structures, carbohydrate binding and anti-HIV 
            activity.
  JOURNAL   FEBS J. 2013 May;280(9):2056-67. doi: 10.1111/febs.12229. Epub 2013 
            Apr 2.
  PUBMED    23480609
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/febs.12229"; journalName: "FEBS J"; date: "2013-05"; 
            volume: "280"; issue: "9"; pages: "2056-67"
COMMENT     Sequence Label: pET15b/BOA
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             46..63
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             73..90
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             109..129
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 taattttgtt taactttaag aaggagatat accatgggca gcagccatca tcatcatcat
       61 cacagcagcg gcctggtgcc gcgcggcagc cacaagactg gccatatgga aaacctgtat
      121 ttccagagtg tcgacatgac caaaacttct aaaggtttta ataacttaca gcacgtgcag
      181 aaccaatggg gtggctcctc cgcaccgtgg cacgaaggcg gtatgtgggt cctgggttgc
      241 cgttccggcc agaacgttgt cgccctgaac attaaatcgg gggatggtgg tcgcaccctg
      301 acaggtacga tgacctacgt tggtgaaggt ccgatcggct tccgtgctac cctgacccag
      361 tctaacacct acgccgtgga aaaccagtgg ggcggctctt cagctccgtg gcacccgggc
      421 ggcacctggg ttatcggttg ccgcgtgaac cagcaagttg ttgctctgga tattgaaagc
      481 ggcgatcagg gcgcgaccct ggcaggtacc atgacctacg ctggcgaagg tccgatcggc
      541 tttaaaagcc agcaggcgga tggcggtgtg tacgcggttg aaaaccagtg gggtggtagc
      601 agcgcgccgt ggcacaacgg tggcgtgtgg gtaatcggtg cacgtgacca ggcggtagta
      661 gcagtttcca tcggttccac cgatagcggc aaaaccctga acggtaacat gacctatgct
      721 ggtgagggtc cgatcggttt caaaggtaat agtgtggccg gtaacaacta cgcggtggaa
      781 aaccagtggg gtggtacctc ggcgccgtgg cacccgggcg gcatttggct gctgggttgc
      841 cgctctggtc agaacgtcgt a
//
