LOCUS       sequence-155454-        2235 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing CHUK , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CHUK_WT
FEATURES             Location/Qualifiers
     source          1..2235
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggagcggc ccccggggct gcggccgggc gcgggcgggc cctgggagat gcgggagcgg
       61 ctgggcaccg gcggcttcgg gaacgtctgt ctgtaccagc atcgggaact tgatctcaaa
      121 atagcaatta agtcttgtcg cctagagcta agtaccaaaa acagagaacg atggtgccat
      181 gaaatccaga ttatgaagaa gttgaaccat gccaatgttg taaaggcctg tgatgttcct
      241 gaagaattga atattttgat tcatgatgtg cctcttctag caatggaata ctgttctgga
      301 ggagatctcc gaaagctgct caacaaacca gaaaattgtt gtggacttaa agaaagccag
      361 atactttctt tactaagtga tatagggtct gggattcgat atttgcatga aaacaaaatt
      421 atacatcgag atctaaaacc tgaaaacata gttcttcagg atgttggtgg aaagataata
      481 cataaaataa ttgatctggg atatgccaaa gatgttgatc aaggaagtct gtgtacatct
      541 tttgtgggaa cactgcagta tctggcccca gagctctttg agaataagcc ttacacagcc
      601 actgttgatt attggagctt tgggaccatg gtatttgaat gtattgctgg atataggcct
      661 tttttgcatc atctgcagcc atttacctgg catgagaaga ttaagaagaa ggatccaaag
      721 tgtatatttg catgtgaaga gatgtcagga gaagttcggt ttagtagcca tttacctcaa
      781 ccaaatagcc tttgtagttt agtagtagaa cccatggaaa actggctaca gttgatgttg
      841 aattgggacc ctcagcagag aggaggacct gttgacctta ctttgaagca gccaagatgt
      901 tttgtattaa tggatcacat tttgaatttg aagatagtac acatcctaaa tatgacttct
      961 gcaaagataa tttcttttct gttaccacct gatgaaagtc ttcattcact acagtctcgt
     1021 attgagcgtg aaactggaat aaatactggt tctcaagaac ttctttcaga gacaggaatt
     1081 tctctggatc ctcggaaacc agcctctcaa tgtgttctag atggagttag aggctgtgat
     1141 agctatatgg tttatttgtt tgataaaagt aaaactgtat atgaagggcc atttgcttcc
     1201 agaagtttat ctgattgtgt aaattatatt gtacaggaca gcaaaataca gcttccaatt
     1261 atacagctgc gtaaagtgtg ggctgaagca gtgcactatg tgtctggact aaaagaagac
     1321 tatagcaggc tctttcaggg acaaagggca gcaatgttaa gtcttcttag atataatgct
     1381 aacttaacaa aaatgaagaa cactttgatc tcagcatcac aacaactgaa agctaaattg
     1441 gagttttttc acaaaagcat tcagcttgac ttggagagat acagcgagca gatgacgtat
     1501 gggatatctt cagaaaaaat gctaaaagca tggaaagaaa tggaagaaaa ggccatccac
     1561 tatgctgagg ttggtgtcat tggatacctg gaggatcaga ttatgtcttt gcatgctgaa
     1621 atcatggagc tacagaagag cccctatgga agacgtcagg gagacttgat ggaatctctg
     1681 gaacagcgtg ccattgatct atataagcag ttaaaacaca gaccttcaga tcactcctac
     1741 agtgacagca cagagatggt gaaaatcatt gtgcacactg tgcagagtca ggaccgtgtg
     1801 ctcaaggagc tgtttggtca tttgagcaag ttgttgggct gtaagcagaa gattattgat
     1861 ctactcccta aggtggaagt ggccctcagt aatatcaaag aagctgacaa tactgtcatg
     1921 ttcatgcagg gaaaaaggca gaaagaaata tggcatctcc ttaaaattgc ctgtacacag
     1981 agttctgccc ggtcccttgt aggatccagt ctagaaggtg cagtaacccc tcagacatca
     2041 gcatggctgc ccccgacttc agcagaacat gatcattctc tgtcatgtgt ggtaactcct
     2101 caagatgggg agacttcagc acaaatgata gaagaaaatt tgaactgcct tggccattta
     2161 agcactatta ttcatgaggc aaatgaggaa cagggcaata gtatgatgaa tcttgattgg
     2221 agttggttaa cagaa
//
