LOCUS       sequence-155469-        2010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing ATF6 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ATF6_WT
FEATURES             Location/Qualifiers
     source          1..2010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..2010
                     /codon_start=1
                     /product="human activating transcription factor 6, an 
                     endoplasmic reticulum (ER) protein involved in the unfolded
                     protein response"
                     /label=ATF6
                     /note="Accumulation of misfolded proteins in the ER leads 
                     to proteolytic cleavage that releases the cytosolic domain 
                     of ATF6, which enters the nucleus and acts as a 
                     transcription factor."
                     /translation="MGEPAGVAGTMESPFSPGLFHRLDEDWDSALFAELGYFTDTDELQ
                     LEAANETYENNFDNLDFDLDLLPWESDIWDINNQICTVKDIKAEPQPLSPASSSYSVSS
                     PRSVDSYSSTQHVPEELDLSSSSQMSPLSLYGENSNSLSSPEPLKEDKPVTGSRNKTEN
                     GLTPKKKIQVNSKPSIQPKPLLLPAAPKTQTNSSVPAKTIIIQTVPTLMPLAKQQPIIS
                     LQPAPTKGQTVLLSQPTVVQLQAPGVLPSAQPVLAVAGGVTQLPNHVVNVVPAPSANSP
                     VNGKLSVTKPVLQSTMRNVGSDIAVLRRQQRMIKNRESACQSRKKKKEYMLGLEARLKA
                     ALSENEQLKKENGTLKRQLDEVVSENQRLKVPSPKRRVVCVMIVLAFIILNYGPMSMLE
                     QDSRRMNPSVSPANQRRHLLGFSAKEAQDTSDGIIQKNSYRYDHSVSNDKALMVLTEEP
                     LLYIPPPPCQPLINTTESLRLNHELRGWVHRHEVERTKSRRMTNNQQKTRILQGALEQG
                     SNSQLMAVQYTETTSSISRNSGSELQVYYASPRSYQDFFEAIRRRGDTFYVVSFRRDHL
                     LLPATTHNKTTRPKMSIVLPAININENVINGQDYEVMMQIDCQVMDTRILHIKSSSVPP
                     YLRDQQRNQTNTFFGSPPAATEATHVVSTIPESLQ"
ORIGIN
        1 atgggggagc cggctggggt tgccggcacc atggagtcac cttttagccc gggactcttt
       61 cacaggctgg atgaagattg ggattctgct ctctttgctg aactcggtta tttcacagac
      121 actgatgagc tgcaattgga agcagcaaat gagacgtatg aaaacaattt tgataatctt
      181 gattttgatt tggatttgtt accttgggag tcagacattt gggacatcaa caaccaaatc
      241 tgtacagtta aagatattaa ggcagaaccc cagccacttt ctccagcctc ctcaagttat
      301 tcagtctcat ctcctcggtc agtggactct tattcttcaa ctcagcatgt tcctgaggag
      361 ttggatttgt cttctagttc tcagatgtct cccctttcct tatatggtga aaactctaat
      421 agtctctctt caccggagcc actgaaggaa gataagcctg tcactggttc taggaacaag
      481 actgaaaatg gactgactcc aaagaaaaaa attcaggtga attcaaaacc ttcaattcag
      541 cccaagcctt tattgcttcc agcagcaccc aagactcaaa caaactccag tgttccagca
      601 aaaaccatca ttattcagac agtaccaacg cttatgccat tggcaaagca gcaaccaatt
      661 atcagtttac aacctgcacc cactaaaggc cagacggttt tgctgtctca gcctactgtg
      721 gtacaacttc aagcacctgg agttctgccc tctgctcagc cagtccttgc tgttgctggg
      781 ggagtcacac agctccctaa tcacgtggtg aatgtggtac cagccccttc agcgaatagc
      841 ccagtgaatg gaaaactttc cgtgactaaa cctgtcctac aaagtaccat gagaaatgtc
      901 ggttcagata ttgctgtgct aaggagacag caacgtatga taaaaaatcg agaatccgct
      961 tgtcagtctc gcaagaagaa gaaagaatat atgctagggt tagaggcgag attaaaggct
     1021 gccctctcag aaaacgagca actgaagaaa gaaaatggaa cactgaagcg gcagctggat
     1081 gaagttgtgt cagagaacca gaggcttaaa gtccctagtc caaagcgaag agttgtctgt
     1141 gtgatgatag tattggcatt tataatactg aactatggac ctatgagcat gttggaacag
     1201 gattccagga gaatgaaccc tagtgtgagc cctgcaaatc aaaggaggca ccttctagga
     1261 ttttctgcta aagaggcaca ggacacatca gatggtatta tccagaaaaa cagctacaga
     1321 tatgatcatt ctgtttcaaa tgacaaagcc ctgatggtgc taactgaaga accattgctt
     1381 tacattcctc cacctccttg tcagccccta attaatacaa cagagtctct caggttaaat
     1441 catgaacttc gaggatgggt tcatagacat gaagtagaaa ggaccaagtc aagaagaatg
     1501 acaaataatc aacagaaaac ccgtattctt cagggtgctc tggaacaggg ctcaaattct
     1561 cagctgatgg ctgttcaata cacagaaacc actagtagta tcagcaggaa ctcagggagt
     1621 gagctacaag tgtattatgc ttcacccaga agttatcaag acttttttga agccatccgc
     1681 agaaggggag acacatttta tgttgtgtca tttcgaaggg atcacctgct gttaccagct
     1741 accacccata acaagaccac aagaccaaaa atgtcaattg tgttaccagc aataaacata
     1801 aatgagaatg tgatcaatgg gcaggactac gaagtgatga tgcagattga ctgtcaggtg
     1861 atggacacca ggatcctcca tatcaaaagt tcgtcggttc ctccttacct ccgagatcag
     1921 cagaggaatc aaaccaacac cttctttggc tcccctcccg cagccacaga ggcaacccac
     1981 gttgtcagca ccatccctga gtcattacaa
//
