LOCUS       sequence-155470-        2529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing AXIN2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_AXIN2_WT
FEATURES             Location/Qualifiers
     source          1..2529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1405..1422
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgagtagcg ctatgttggt gacttgcctc ccggacccca gcagcagctt ccgtgaggat
       61 gccccgcggc ccccagtgcc aggggaagaa ggggagaccc caccgtgtca gccaggggtg
      121 ggcaagggcc aggtcaccaa acccatgtct gtctcttcca acaccaggcg gaacgaagat
      181 gggttggggg agccggaggg gcgggcatct ccggattccc ctctgacccg gtggaccaag
      241 tccttacact ccttattggg cgatcaagac ggtgcttacc tgttccgaac tttcctggag
      301 agggagaaat gcgtggatac cttagacttc tggtttgcct gcaatggatt caggcagatg
      361 aacctgaagg ataccaaaac tttacgagta gccaaagcga tctacaaaag gtacattgag
      421 aacaacagca ttgtctccaa gcagctgaag cctgccacca agacctacat aagagatggc
      481 atcaagaagc agcagattga ttccatcatg tttgaccagg cgcagaccga gatccagtcg
      541 gtgatggagg aaaatgccta ccagatgttt ttgacttctg atatatacct cgaatatgtg
      601 aggagtgggg gagaaaacac agcttacatg agtaatgggg gactcgggag cctaaaggtc
      661 gtgtgtggct atctccccac cttgaatgaa gaagaggagt ggacttgtgc cgacttcaag
      721 tgcaaacttt cgccaaccgt ggttggcttg tccagcaaaa ctctgagggc cacggcgagt
      781 gtgaggtcca cggaaactgt tgacagtgga tacaggtcct tcaagaggag cgatcctgtt
      841 aatccttatc acataggttc tggctatgtc tttgcaccag ccaccagcgc caacgacagt
      901 gagatatcca gtgatgcgct gacggatgat tccatgtcca tgacggacag cagtgtagat
      961 ggaattcctc cttatcgtgt gggcagtaag aaacagctcc agagagaaat gcatcgcagt
     1021 gtgaaggcca atggccaagt gtctctacct catttcccga gaacccaccg cctgcccaag
     1081 gagatgaccc ccgtggaacc cgccaccttt gcagctgagc tgatctcgag gctggaaaag
     1141 ctgaagctgg agttggagag ccgccacagc ctggaggagc gcctgcagca gatccgagag
     1201 gatgaagaga gagagggctc cgagctcaca ctcaattcgc gggagggggc gcccacgcag
     1261 caccccctct ccctactgcc ctccggcagc tacgaggaag acccgcagac gatactggac
     1321 gatcacctgt ccagggtcct caagacccct ggctgccagt ctccgggcgt aggccgctat
     1381 agccctcgct cccgctcccc ggaccaccac caccaccacc attcgcagta ccactccctg
     1441 ctcccgcccg gtggcaagct gcctcccgcg gccgcctcgc cgggcgcctg ccccctcctc
     1501 gggggcaaag gctttgtgac caagcagacg acgaagcatg tccaccacca ctacatccac
     1561 caccatgccg tccccaagac caaggaggag atcgaggcgg aggccacgca gcgggtgcac
     1621 tgcttctgcc ctgggggcag cgagtattac tgctactcga aatgcaaaag ccactccaag
     1681 gctccggaaa ccatgcccag cgagcagttt ggcggcagca gaggcagtac cttgcccaaa
     1741 cgcaatggga aaggcacgga gccgggcctg gccctgcccg ccagggaagg aggggccccc
     1801 ggcggagctg gggccctgca gcttccccgg gaggaaggag acaggtcgca ggatgtctgg
     1861 cagtggatgc tggagagtga gcggcagagc aagcccaagc cccatagtgc ccaaagcaca
     1921 aaaaaggcct accccttgga gtctgcccgc tcgtctccag gcgaacgagc cagccggcac
     1981 catctgtggg ggggcaacag cgggcacccc cgcaccaccc cccgtgccca cctgttcacc
     2041 caggaccctg cgatgcctcc cctgacccca cccaacacgc tggctcagct ggaggaggcc
     2101 tgtcgcaggc tagctgaggt gtcgaagccc ccaaagcagc ggtgctgtgt ggccagtcag
     2161 cagagggaca ggaatcattc ggccactgtt cagacgggag ccacaccctt ctccaatcca
     2221 agcctggctc cagaagatca caaagagcca aagaaactgg caggtgtcca cgcgctccag
     2281 gccagtgagt tggttgtcac ttactttttc tgtggggaag aaattccata ccggaggatg
     2341 ctgaaggctc agagcttgac cctgggccac tttaaagagc agctcagcaa aaagggaaat
     2401 tataggtatt acttcaaaaa agcaagcgat gagtttgcct gtggagcggt gtttgaggag
     2461 atctgggagg atgagacggt gctcccgatg tatgaaggcc ggattctggg caaagtggag
     2521 cggatcgat
//
