LOCUS       sequence-155547-        2343 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing CTNNB1 , part of the Target 
            Accelerator Plasmid Collection. (Please note this plasmid contains 
            S45A mutation, not T45A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CTNNB1_p.S33A_S37A_T41A_T45A
FEATURES             Location/Qualifiers
     source          1..2343
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..2343
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDAGIHAGATATAPA
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     CDS             1..2343
                     /codon_start=1
                     /gene="Homo sapiens CTNNB1"
                     /product="human cadherin-associated protein that also plays
                     a role in the canonical Wnt signaling pathway"
                     /label=beta-catenin
                     /note="also known as catenin beta-1"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDAGIHAGATATAPA
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
ORIGIN
        1 atggctactc aagctgattt gatggagttg gacatggcca tggaaccaga cagaaaagcg
       61 gctgttagtc actggcagca acagtcttac ctggacgctg gaatccatgc tggtgccact
      121 gccacagctc ctgctctgag tggtaaaggc aatcctgagg aagaggatgt ggatacctcc
      181 caagtcctgt atgagtggga acagggattt tctcagtcct tcactcaaga acaagtagct
      241 gatattgatg gacagtatgc aatgactcga gctcagaggg tacgagctgc tatgttccct
      301 gagacattag atgagggcat gcagatccca tctacacagt ttgatgctgc tcatcccact
      361 aatgtccagc gtttggctga accatcacag atgctgaaac atgcagttgt aaacttgatt
      421 aactatcaag atgatgcaga acttgccaca cgtgcaatcc ctgaactgac aaaactgcta
      481 aatgacgagg accaggtggt ggttaataag gctgcagtta tggtccatca gctttctaaa
      541 aaggaagctt ccagacacgc tatcatgcgt tctcctcaga tggtgtctgc tattgtacgt
      601 accatgcaga atacaaatga tgtagaaaca gctcgttgta ccgctgggac cttgcataac
      661 ctttcccatc atcgtgaggg cttactggcc atctttaagt ctggaggcat tcctgccctg
      721 gtgaaaatgc ttggttcacc agtggattct gtgttgtttt atgccattac aactctccac
      781 aaccttttat tacatcaaga aggagctaaa atggcagtgc gtttagctgg tgggctgcag
      841 aaaatggttg ccttgctcaa caaaacaaat gttaaattct tggctattac gacagactgc
      901 cttcaaattt tagcttatgg caaccaagaa agcaagctca tcatactggc tagtggtgga
      961 ccccaagctt tagtaaatat aatgaggacc tatacttacg aaaaactact gtggaccaca
     1021 agcagagtgc tgaaggtgct atctgtctgc tctagtaata agccggctat tgtagaagct
     1081 ggtggaatgc aagctttagg acttcacctg acagatccaa gtcaacgtct tgttcagaac
     1141 tgtctttgga ctctcaggaa tctttcagat gctgcaacta aacaggaagg gatggaaggt
     1201 ctccttggga ctcttgttca gcttctgggt tcagatgata taaatgtggt cacctgtgca
     1261 gctggaattc tttctaacct cacttgcaat aattataaga acaagatgat ggtctgccaa
     1321 gtgggtggta tagaggctct tgtgcgtact gtccttcggg ctggtgacag ggaagacatc
     1381 actgagcctg ccatctgtgc tcttcgtcat ctgaccagcc gacaccaaga agcagagatg
     1441 gcccagaatg cagttcgcct tcactatgga ctaccagttg tggttaagct cttacaccca
     1501 ccatcccact ggcctctgat aaaggctact gttggattga ttcgaaatct tgccctttgt
     1561 cccgcaaatc atgcaccttt gcgtgagcag ggtgccattc cacgactagt tcagttgctt
     1621 gttcgtgcac atcaggatac ccagcgccgt acgtccatgg gtgggacaca gcagcaattt
     1681 gtggaggggg tccgcatgga agaaatagtt gaaggttgta ccggagccct tcacatccta
     1741 gctcgggatg ttcacaaccg aattgttatc agaggactaa ataccattcc attgtttgtg
     1801 cagctgcttt attctcccat tgaaaacatc caaagagtag ctgcaggggt cctctgtgaa
     1861 cttgctcagg acaaggaagc tgcagaagct attgaagctg agggagccac agctcctctg
     1921 acagagttac ttcactctag gaatgaaggt gtggcgacat atgcagctgc tgttttgttc
     1981 cgaatgtctg aggacaagcc acaagattac aagaaacggc tttcagttga gctgaccagc
     2041 tctctcttca gaacagagcc aatggcttgg aatgagactg ctgatcttgg acttgatatt
     2101 ggtgcccagg gagaacccct tggatatcgc caggatgatc ctagctatcg ttcttttcac
     2161 tctggtggat atggccagga tgccttgggt atggacccca tgatggaaca tgagatgggt
     2221 ggccaccacc ctggtgctga ctatccagtt gatgggctgc cagatctggg gcatgcccag
     2281 gacctcatgg atgggctgcc tccaggtgac agcaatcagc tggcctggtt tgatactgac
     2341 ctg
//
