LOCUS       sequence-155550-         576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing RAC1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_RAC1_p.T17N
FEATURES             Location/Qualifiers
     source          1..576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..48,49..51,52..576)
                     /codon_start=1
                     /product="dominant negative mutant of human Rac family 
                     small GTPase 1"
                     /label=Rac1 (T17N)
                     /translation="MQAIKCVVVGDGAVGKNCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
ORIGIN
        1 atgcaggcca tcaagtgtgt ggtggtggga gacggagctg taggtaaaaa ctgcctactg
       61 atcagttaca caaccaatgc atttcctgga gaatatatcc ctactgtctt tgacaattat
      121 tctgccaatg ttatggtaga tggaaaaccg gtgaatctgg gcttatggga tacagctgga
      181 caagaagatt atgacagatt acgcccccta tcctatccgc aaacagatgt gttcttaatt
      241 tgcttttccc ttgtgagtcc tgcatcattt gaaaatgtcc gtgcaaagtg gtatcctgag
      301 gtgcggcacc actgtcccaa cactcccatc atcctagtgg gaactaaact tgatcttagg
      361 gatgataaag acacgatcga gaaactgaag gagaagaagc tgactcccat cacctatccg
      421 cagggtctag ccatggctaa ggagattggt gctgtaaaat acctggagtg ctcggcgctc
      481 acacagcgag gcctcaagac agtgtttgac gaagcgatcc gagcagtcct ctgcccgcct
      541 cccgtgaaga agaggaagag aaaatgcctg ctgttg
//
