LOCUS       sequence-155553-         579 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing RHOA , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_RHOA_p.T19N
FEATURES             Location/Qualifiers
     source          1..579
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1..54,55..57,58..579)
                     /codon_start=1
                     /product="dominant negative mutant of human Rho family 
                     small GTPase RhoA"
                     /label=RhoA (T19N)
                     /translation="MAAIRKKLVIVGDGACGKNCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
ORIGIN
        1 atggctgcca tccggaagaa actggtgatt gttggtgatg gagcctgtgg aaagaactgc
       61 ttgctcatag tcttcagcaa ggaccagttc ccagaggtgt atgtgcccac agtgtttgag
      121 aactatgtgg cagatatcga ggtggatgga aagcaggtag aattggcttt gtgggacaca
      181 gctgggcagg aagattatga tcgcctgagg cccctctcct acccagatac cgatgttata
      241 ctgatgtgtt tttccatcga cagccctgat agtttagaaa acatcccaga aaagtggacc
      301 ccagaagtca agcatttctg tcccaacgtg cccatcatcc tggttgggaa taagaaggat
      361 cttcggaatg atgagcacac aaggcgggag ctagccaaga tgaagcagga gccggtgaaa
      421 cctgaagaag gcagagatat ggcaaacagg attggcgctt ttgggtacat ggagtgttca
      481 gcaaagacca aagatggagt gagagaggtt tttgaaatgg ctacgagagc tgctctgcaa
      541 gctagacgtg ggaagaaaaa atctgggtgc cttgtcttg
//
