LOCUS       sequence-155557-        2298 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing BRAF , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_BRAF_p.V600E
FEATURES             Location/Qualifiers
     source          1..2298
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggcggcgc tgagcggtgg cggtggtggc ggcgctgagc cgggccaggc tctgttcaac
       61 ggggacatgg agcccgaggc cggcgccggc gccggcgccg cggcctcttc ggctgcggac
      121 cctgccattc cggaggaggt gtggaatatc aaacaaatga ttaagttgac acaggaacat
      181 atagaggccc tattggacaa atttggtggg gagcataatc caccatcaat atatctggag
      241 gcctatgaag aatacaccag caagctagat gcactccaac aaagagaaca acagttattg
      301 gaatctctgg ggaacggaac tgatttttct gtttctagct ctgcatcaat ggataccgtt
      361 acatcttctt cctcttctag cctttcagtg ctaccttcat ctctttcagt ttttcaaaat
      421 cccacagatg tggcacggag caaccccaag tcaccacaaa aacctatcgt tagagtcttc
      481 ctgcccaaca aacagaggac agtggtacct gcaaggtgtg gagttacagt ccgagacagt
      541 ctaaagaaag cactgatgat gagaggtcta ataccagagt gctgtgctgt ttacagaatt
      601 caggatggag agaagaaacc aattggttgg gacactgata tttcctggct tactggagaa
      661 gaattgcatg tggaagtgtt ggagaatgtt ccacttacaa cacacaactt tgtacgaaaa
      721 acgtttttca ccttagcatt ttgtgacttt tgtcgaaagc tgcttttcca gggtttccgc
      781 tgtcaaacat gtggttataa atttcaccag cgttgtagta cagaagttcc actgatgtgt
      841 gttaattatg accaacttga tttgctgttt gtctccaagt tctttgaaca ccacccaata
      901 ccacaggaag aggcgtcctt agcagagact gccctaacat ctggatcatc cccttccgca
      961 cccgcctcgg actctattgg gccccaaatt ctcaccagtc cgtctccttc aaaatccatt
     1021 ccaattccac agcccttccg accagcagat gaagatcatc gaaatcaatt tgggcaacga
     1081 gaccgatcct catcagctcc caatgtgcat ataaacacaa tagaacctgt caatattgat
     1141 gacttgatta gagaccaagg atttcgtggt gatggaggat caaccacagg tttgtctgct
     1201 accccccctg cctcattacc tggctcacta actaacgtga aagccttaca gaaatctcca
     1261 ggacctcagc gagaaaggaa gtcatcttca tcctcagaag acaggaatcg aatgaaaaca
     1321 cttggtagac gggactcgag tgatgattgg gagattcctg atgggcagat tacagtggga
     1381 caaagaattg gatctggatc atttggaaca gtctacaagg gaaagtggca tggtgatgtg
     1441 gcagtgaaaa tgttgaatgt gacagcacct acacctcagc agttacaagc cttcaaaaat
     1501 gaagtaggag tactcaggaa aacacgacat gtgaatatcc tactcttcat gggctattcc
     1561 acaaagccac aactggctat tgttacccag tggtgtgagg gctccagctt gtatcaccat
     1621 ctccatatca ttgagaccaa atttgagatg atcaaactta tagatattgc acgacagact
     1681 gcacagggca tggattactt acacgccaag tcaatcatcc acagagacct caagagtaat
     1741 aatatatttc ttcatgaaga cctcacagta aaaataggtg attttggtct agctacagag
     1801 aaatctcgat ggagtgggtc ccatcagttt gaacagttgt ctggatccat tttgtggatg
     1861 gcaccagaag tcatcagaat gcaagataaa aatccataca gctttcagtc agatgtatat
     1921 gcatttggga ttgttctgta tgaattgatg actggacagt taccttattc aaacatcaac
     1981 aacagggacc agataatttt tatggtggga cgaggatacc tgtctccaga tctcagtaag
     2041 gtacggagta actgtccaaa agccatgaag agattaatgg cagagtgcct caaaaagaaa
     2101 agagatgaga gaccactctt tccccaaatt ctcgcctcta ttgagctgct ggcccgctca
     2161 ttgccaaaaa ttcaccgcag tgcatcagaa ccctccttga atcgggctgg tttccaaaca
     2221 gaggatttta gtctatatgc ttgtgcttct ccaaaaacac ccatccaggc agggggatat
     2281 ggtgcgtttc ctgtccac
//
