LOCUS       sequence-155572-         492 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing CDKN1A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CDKN1A_WT
FEATURES             Location/Qualifiers
     source          1..492
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..492
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRK
                     P"
ORIGIN
        1 atgtcagaac cggctgggga tgtccgtcag aacccatgcg gcagcaaggc ctgccgccgc
       61 ctcttcggcc cagtggacag cgagcagctg agccgcgact gtgatgcgct aatggcgggc
      121 tgcatccagg aggcccgtga gcgatggaac ttcgactttg tcaccgagac accactggag
      181 ggtgacttcg cctgggagcg tgtgcggggc cttggcctgc ccaagctcta ccttcccacg
      241 gggccccggc gaggccggga tgagttggga ggaggcaggc ggcctggcac ctcacctgct
      301 ctgctgcagg ggacagcaga ggaagaccat gtggacctgt cactgtcttg tacccttgtg
      361 cctcgctcag gggagcaggc tgaagggtcc ccaggtggac ctggagactc tcagggtcga
      421 aaacggcggc agaccagcat gacagatttc taccactcca aacgccggct gatcttctcc
      481 aagaggaagc cc
//
