LOCUS       sequence-155585-        3726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing IRS1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_IRS1_WT
FEATURES             Location/Qualifiers
     source          1..3726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3085..3094
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgagcc ctccggagag cgatggcttc tcggacgtgc gcaaggtggg ctacctgcgc
       61 aaacccaaga gcatgcacaa acgcttcttc gtactgcgcg cggccagcga ggctgggggc
      121 ccggcgcgcc tcgagtacta cgagaacgag aagaagtggc ggcacaagtc gagcgccccc
      181 aaacgctcga tcccccttga gagctgcttc aacatcaaca agcgggctga ctccaagaac
      241 aagcacctgg tggctctcta cacccgggac gagcactttg ccatcgcggc ggacagcgag
      301 gccgagcaag acagctggta ccaggctctc ctacagctgc acaaccgtgc taagggccac
      361 cacgacggag ctgcggccct cggggcggga ggtggtgggg gcagctgcag cggcagctcc
      421 ggccttggtg aggctgggga ggacttgagc tacggtgacg tgcccccagg acccgcattc
      481 aaagaggtct ggcaagtgat cctgaagccc aagggcctgg gtcagacaaa gaacctgatt
      541 ggtatctacc gcctttgcct gaccagcaag accatcagct tcgtgaagct gaactcggag
      601 gcagcggccg tggtgctgca gctgatgaac atcaggcgct gtggccactc ggaaaacttc
      661 ttcttcatcg aggtgggccg ttctgccgtg acggggcccg gggagttctg gatgcaggtg
      721 gatgactctg tggtggccca gaacatgcac gagaccatcc tggaggccat gcgggccatg
      781 agtgatgagt tccgccctcg cagcaagagc cagtcctcgt ccaactgctc taaccccatc
      841 agcgtccccc tgcgccggca ccatctcaac aatcccccgc ccagccaggt ggggctgacc
      901 cgccgatcac gcactgagag catcaccgcc acctccccgg ccagcatggt gggcgggaag
      961 ccaggctcct tccgtgtccg cgcctccagt gacggcgaag gcaccatgtc ccgcccagcc
     1021 tcggtggacg gcagccctgt gagtcccagc accaacagaa cccacgccca ccggcatcgg
     1081 ggcagcgccc ggctgcaccc cccgctcaac cacagccgct ccatccccat gccggcttcc
     1141 cgctgctcgc cttcggccac cagcccggtc agtctgtcgt ccagtagcac cagtggccat
     1201 ggctccacct cggattgtct cttcccacgg cgatctagtg cttcggtgtc tggttccccc
     1261 agcgatggcg gtttcatctc ctcggatgag tatggctcca gtccctgcga tttccggagt
     1321 tccttccgca gtgtcactcc ggattccctg ggccacaccc caccagcccg cggtgaggag
     1381 gagctaagca actatatctg catgggtggc aaggggccct ccaccctgac cgcccccaac
     1441 ggtcactaca ttttgtctcg gggtggcaat ggccaccgct gcaccccagg aacaggcttg
     1501 ggcacgagtc cagccttggc tggggatgaa gcagccagtg ctgcagatct ggataatcgg
     1561 ttccgaaaga gaactcactc ggcaggcaca tcccctacca ttacccacca gaagaccccg
     1621 tcccagtcct cagtggcttc cattgaggag tacacagaga tgatgcctgc ctacccacca
     1681 ggaggtggca gtggaggccg actgccggga cacaggcact ccgccttcgt gcccacccgc
     1741 tcctacccag aggagggtct ggaaatgcac cccttggagc gtcggggggg gcaccaccgc
     1801 ccagacagct ccaccctcca cacggatgat ggctacatgc ccatgtcccc aggggtggcc
     1861 ccagtgccca gtggccgaaa gggcagtgga gactatatgc ccatgagccc caagagcgta
     1921 tctgccccac agcagatcat caatcccatc agacgccatc cccagagagt ggaccccaat
     1981 ggctacatga tgatgtcccc cagcggtggc tgctctcctg acattggagg tggccccagc
     2041 agcagcagca gcagcagcaa cgccgtccct tccgggacca gctatggaaa gctgtggaca
     2101 aacggggtag ggggccacca ctctcatgtc ttgcctcacc ccaaaccccc agtggagagc
     2161 agcggtggta agctcttacc ttgcacaggt gactacatga acatgtcacc agtgggggac
     2221 tccaacacca gcagcccctc cgactgctac tacggccctg aggaccccca gcacaagcca
     2281 gtcctctcct actactcatt gccaagatcc tttaagcaca cccagcgccc cggggagccg
     2341 gaggagggtg cccggcatca gcacctccgc ctttccacta gctctggtcg ccttctctat
     2401 gctgcaacag cagatgattc ttcctcttcc accagcagcg acagcctggg tgggggatac
     2461 tgcggggcta ggctggagcc cagccttcca catccccacc atcaggttct gcagccccat
     2521 ctgcctcgaa aggtggacac agctgctcag accaatagcc gcctggcccg gcccacgagg
     2581 ctgtccctgg gggatcccaa ggccagcacc ttacctcggg cccgagagca gcagcagcag
     2641 cagcagccct tgctgcaccc tccagagccc aagagcccgg gggaatatgt caatattgaa
     2701 tttgggagtg atcagtctgg ctacttgtct ggcccggtgg ctttccacag ctcaccttct
     2761 gtcaggtgtc catcccagct ccagccagct cccagagagg aagagactgg cactgaggag
     2821 tacatgaaga tggacctggg gccgggccgg agggcagcct ggcaggagag cactggggtc
     2881 gagatgggca gactgggccc tgcacctccc ggggctgcta gcatttgcag gcctacccgg
     2941 gcagtgccca gcagccgggg tgactacatg accatgcaga tgagttgtcc ccgtcagagc
     3001 tacgtggaca cctcgccagc tgcccctgta agctatgctg acatgcgaac aggcattgct
     3061 gcagaggagg tgagcctgcc cagggccacc atggctgctg cctcctcatc ctcagcagcc
     3121 tctgcttccc cgactgggcc tcaaggggca gcagagctgg ctgcccactc gtccctgctg
     3181 gggggcccac aaggacctgg gggcatgagc gccttcaccc gggtgaacct cagtcctaac
     3241 cgcaaccaga gtgccaaagt gatccgtgca gacccacaag ggtgccggcg gaggcatagc
     3301 tccgagactt tctcctcaac acccagtgcc acccgggtgg gcaacacagt gccctttgga
     3361 gcgggggcag cagtaggggg cggtggcggt agcagcagca gcagcgagga tgtgaaacgc
     3421 cacagctctg cttcctttga gaatgtgtgg ctgaggcctg gggagcttgg gggagccccc
     3481 aaggagccag ccaaactgtg tggggctgct gggggtttgg agaatggtct taactacata
     3541 gacctggatt tggtcaagga cttcaaacag tgccctcagg agtgcacccc tgaaccgcag
     3601 cctcccccac ccccaccccc tcatcaaccc ctgggcagcg gtgagagcag ctccacccgc
     3661 cgctcaagtg aggatttaag cgcctatgcc agcatcagtt tccagaagca gccagaggac
     3721 cgtcag
//
