LOCUS       sequence-155591-        1635 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing PAK1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PAK1_WT
FEATURES             Location/Qualifiers
     source          1..1635
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(533..550)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtcaaata acggcctaga cattcaagac aaacccccag cccctccgat gagaaatacc
       61 agcactatga ttggagccgg cagcaaagat gctggaaccc taaaccatgg ttctaaacct
      121 ctgcctccaa acccagagga gaagaaaaag aaggaccgat tttaccgatc cattttacct
      181 ggagataaaa caaataaaaa gaaagagaaa gagcggccag agatttctct cccttcagat
      241 tttgaacaca caattcatgt cggttttgat gctgtcacag gggagtttac gggaatgcca
      301 gagcagtggg cccgcttgct tcagacatca aatatcacta agtcggagca gaagaaaaac
      361 ccgcaggctg ttctggatgt gttggagttt tacaactcga agaagacatc caacagccag
      421 aaatacatga gctttacaga taagtcagct gaggattaca attcttctaa tgccttgaat
      481 gtgaaggctg tgtctgagac tcctgcagtg ccaccagttt cagaagatga ggatgatgat
      541 gatgatgatg ctaccccacc accagtgatt gctccacgcc cagagcacac aaaatctgta
      601 tacacacggt ctgtgattga accacttcct gtcactccaa ctcgggacgt ggctacatct
      661 cccatttcac ctactgaaaa taacaccact ccaccagatg ctttgacccg gaatactgag
      721 aagcagaaga agaagcctaa aatgtctgat gaggagatct tggagaaatt acgaagcata
      781 gtgagtgtgg gcgatcctaa gaagaaatat acacggtttg agaagattgg acaaggtgct
      841 tcaggcaccg tgtacacagc aatggatgtg gccacaggac aggaggtggc cattaagcag
      901 atgaatcttc agcagcagcc caagaaagag ctgattatta atgagatcct ggtcatgagg
      961 gaaaacaaga acccaaacat tgtgaattac ttggacagtt acctcgtggg agatgagctg
     1021 tgggttgtta tggaatactt ggctggaggc tccttgacag atgtggtgac agaaacttgc
     1081 atggatgaag gccaaattgc agctgtgtgc cgtgagtgtc tgcaggctct ggagttcttg
     1141 cattcgaacc aggtcattca cagagacatc aagagtgaca atattctgtt gggaatggat
     1201 ggctctgtca agctaactga ctttggattc tgtgcacaga taaccccaga gcagagcaaa
     1261 cggagcacca tggtaggaac cccatactgg atggcaccag aggttgtgac acgaaaggcc
     1321 tatgggccca aggttgacat ctggtccctg ggcatcatgg ccatcgaaat gattgaaggg
     1381 gagcctccat acctcaatga aaaccctctg agagccttgt acctcattgc caccaatggg
     1441 accccagaac ttcagaaccc agagaagctg tcagctatct tccgggactt tctgaaccgc
     1501 tgtctcgaga tggatgtgga gaagagaggt tcagctaaag agctgctaca gcatcaattc
     1561 ctgaagattg ccaagcccct ctccagcctc actccactga ttgctgcagc taaggaggca
     1621 acaaagaaca atcac
//
