LOCUS       sequence-155592-        3210 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing PIK3CB , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PIK3CB_WT
FEATURES             Location/Qualifiers
     source          1..3210
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             209..217
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(1516..1527)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atgtgcttca gtttcataat gcctcctgct atggcagaca tccttgacat ctgggcggtg
       61 gattcacaga tagcatctga tggctccata cctgtggatt tccttttgcc cactgggatt
      121 tatatccagt tggaggtacc tcgggaagct accatttctt atattaagca gatgttatgg
      181 aagcaagttc acaattaccc aatgttcaac ctccttatgg atattgactc ctatatgttt
      241 gcatgtgtga atcagactgc tgtatatgag gagcttgaag atgaaacacg aagactctgt
      301 gatgtcagac cttttcttcc agttctcaaa ttagtgacaa gaagttgtga cccaggggaa
      361 aaattagact caaaaattgg agtccttata ggaaaaggtc tgcatgaatt tgattccttg
      421 aaggatcctg aagtaaatga atttcgaaga aaaatgcgca aattcagcga ggaaaaaatc
      481 ctgtcacttg tgggattgtc ttggatggac tggctaaaac aaacatatcc accagagcat
      541 gaaccatcca tccctgaaaa cttagaagat aaactttatg ggggaaagct catcgtagct
      601 gttcattttg aaaactgcca ggacgtgttt agctttcaag tgtctcctaa tatgaatcct
      661 atcaaagtaa atgaattggc aatccaaaaa cgtttgacta ttcatgggaa ggaagatgaa
      721 gttagcccct atgattatgt gttgcaagtc agcgggagag tagaatatgt ttttggtgat
      781 catccactaa ttcagttcca gtatatccgg aactgtgtga tgaacagagc cctgccccat
      841 tttatacttg tggaatgctg caagatcaag aaaatgtatg aacaagaaat gattgccata
      901 gaggctgcca taaatcgaaa ttcatctaat cttcctcttc cattaccacc aaagaaaaca
      961 cgaattattt ctcatgtttg ggaaaataac aaccctttcc aaattgtctt ggttaaggga
     1021 aataaactta acacagagga aactgtaaaa gttcatgtca gggctggtct ttttcatggt
     1081 actgagctcc tgtgtaaaac catcgtaagc tcagaggtat cagggaaaaa tgatcatatt
     1141 tggaatgaac cactggaatt tgatattaat atttgtgact taccaagaat ggctcgatta
     1201 tgttttgctg tttatgcagt tttggataaa gtaaaaacga agaaatcaac gaaaactatt
     1261 aatccctcta aatatcagac catcaggaaa gctggaaaag tgcattatcc tgtagcgtgg
     1321 gtaaatacga tggtttttga ctttaaagga caattgagaa ctggagacat aatattacac
     1381 agctggtctt catttcctga tgaactcgaa gaaatgttga atccaatggg aactgttcaa
     1441 acaaatccat atactgaaaa tgcaacagct ttgcatgtta aatttccaga gaataaaaaa
     1501 caaccttatt attaccctcc cttcgataag attattgaaa aggcagctga gattgcaagc
     1561 agtgatagtg ctaatgtgtc aagtcgaggt ggaaaaaagt ttcttcctgt attgaaagaa
     1621 atcttggaca gggatccctt gtctcaactg tgtgaaaatg aaatggatct tatttggact
     1681 ttgcgacaag actgccgaga gattttccca caatcactgc caaaattact gctgtcaatc
     1741 aagtggaata aacttgagga tgttgctcag cttcaggcgc tgcttcagat ttggcctaaa
     1801 ctgccccccc gggaggccct agagcttctg gatttcaact atccagacca gtacgttcga
     1861 gaatatgctg taggctgcct gcgacagatg agtgatgaag aactttctca atatctttta
     1921 caactggtgc aagtgttaaa atatgagcct tttcttgatt gtgccctctc tagattccta
     1981 ttagaaagag cacttggtaa tcggaggata gggcagtttc tattttggca tcttaggtca
     2041 gaagtgcaca ttcctgctgt ctcagtacaa tttggtgtca tccttgaagc atactgccgg
     2101 ggaagtgtgg ggcacatgaa agtgctttct aagcaggttg aagcactcaa taagttaaaa
     2161 actttaaata gtttaatcaa actgaatgcc gtgaagttaa acagagccaa agggaaggag
     2221 gccatgcata cctgtttaaa acagagtgct taccgggaag ccctctctga cctgcagtca
     2281 cccctgaacc catgtgttat cctctcagaa ctctatgttg aaaagtgcaa atacatggat
     2341 tccaaaatga agcctttgtg gctggtatac aataacaagg tatttggtga ggattcagtt
     2401 ggagtgattt ttaaaaatgg tgatgattta cgacaggata tgttgacact ccaaatgttg
     2461 cgcttgatgg atttactctg gaaagaagct ggtttggatc ttcggatgtt gccttatggc
     2521 tgtttagcaa caggagatcg ctctggcctc attgaagttg tgagcacctc tgaaacaatt
     2581 gctgacattc agctgaacag tagcaatgtg gctgctgcag cagccttcaa caaagatgcc
     2641 cttctgaact ggcttaaaga atacaactct ggggatgacc tggaccgagc cattgaggaa
     2701 tttacactgt cctgtgctgg ctactgtgta gcttcttatg tccttgggat tggtgacaga
     2761 catagtgaca acatcatggt caaaaaaact ggccagctct tccacattga ctttggacat
     2821 attcttggaa atttcaaatc taagtttggc attaaaaggg agcgagtgcc ttttattctt
     2881 acctatgatt tcatccatgt cattcaacaa ggaaaaacag gaaatacaga aaagtttggc
     2941 cggttccgcc agtgttgtga ggatgcatat ctgattttac gacggcatgg gaatctcttc
     3001 atcactctct ttgcgctgat gttgactgca gggcttcctg aactcacatc agtcaaagat
     3061 atacagtatc ttaaggactc tcttgcatta gggaagagtg aagaagaagc actcaaacag
     3121 tttaagcaaa aatttgatga ggcgctcagg gaaagctgga ctactaaagt gaactggatg
     3181 gcccacacag ttcggaaaga ctacagatct
//
