LOCUS       sequence-155598-        2211 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing PRKCE , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_PRKCE_WT
FEATURES             Location/Qualifiers
     source          1..2211
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             648..659
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 atggtagtgt tcaatggcct tcttaagatc aaaatctgcg aggccgtgag cttgaagccc
       61 acagcctggt cgctgcgcca tgcggtggga ccccggccgc agactttcct tctcgacccc
      121 tacattgccc tcaatgtgga cgactcgcgc atcggccaaa cggccaccaa gcagaagacc
      181 aacagcccgg cctggcacga cgagttcgtc accgatgtgt gcaacggacg caagatcgag
      241 ctggctgtct ttcacgatgc ccccataggc tacgacgact tcgtggccaa ctgcaccatc
      301 cagtttgagg agctgctgca gaacgggagc cgccacttcg aggactggat tgatctggag
      361 ccagaaggaa gagtgtatgt gatcatcgat ctctcagggt cgtcgggtga agcccctaaa
      421 gacaatgaag agcgtgtgtt cagggaacgc atgcggccga ggaagcggca gggggccgtc
      481 aggcgcaggg tccatcaggt caacggccac aagttcatgg ccacctatct tcggcagccc
      541 acctactgct cccattgcag agacttcatc tggggtgtca taggaaagca gggataccag
      601 tgtcaagtct gcacctgcgt ggtccacaag cggtgccacg agctcataat cacaaagtgt
      661 gctgggttaa agaagcagga gacccccgac caggtgggct cccagcggtt cagcgtcaac
      721 atgccccaca agttcggtat ccacaactac aaggtcccta ccttctgcga tcactgtggg
      781 tccctgctct ggggactctt gcggcagggt ttgcagtgta aagtctgcaa aatgaatgtt
      841 caccgtcgat gtgagaccaa cgtggctccc aactgtggag tggatgccag aggaatcgcc
      901 aaagtactgg ccgacctggg cgttacccca gacaaaatca ccaacagcgg ccagagaagg
      961 aaaaagctca ttgctggtgc cgagtccccg cagcctgctt ctggaagctc accatctgag
     1021 gaagatcgat ccaagtcagc acccacctcc ccttgtgacc aggaaataaa agaacttgag
     1081 aacaacattc ggaaagcctt gtcatttgac aaccgaggag aggagcaccg ggcagcatcg
     1141 tctcctgatg gccagctgat gagccccggt gagaatggcg aagtccggca aggccaggcc
     1201 aagcgcctgg gcctggatga gttcaacttc atcaaggtgt tgggcaaagg cagctttggc
     1261 aaggtcatgt tggcagaact caagggcaaa gatgaagtat atgctgtgaa ggtcttaaag
     1321 aaggacgtca tccttcagga tgatgacgtg gactgcacaa tgacagagaa gaggattttg
     1381 gctctggcac ggaaacaccc gtaccttacc caactctact gctgcttcca gaccaaggac
     1441 cgcctctttt tcgtcatgga atatgtaaat ggtggagacc tcatgtttca gattcagcgc
     1501 tcccgaaaat tcgacgagcc tcgttcacgg ttctatgctg cagaggtcac atcggccctc
     1561 atgttcctcc accagcatgg agtcatctac agggatttga aactggacaa catccttctg
     1621 gatgcagaag gtcactgcaa gctggctgac ttcgggatgt gcaaggaagg gattctgaat
     1681 ggtgtgacga ccaccacgtt ctgtgggact cctgactaca tagctcctga gatcctgcag
     1741 gagttggagt atggcccctc cgtggactgg tgggccctgg gggtgctgat gtacgagatg
     1801 atggctggac agcctccctt tgaggccgac aatgaggacg acctatttga gtccatcctc
     1861 catgacgacg tgctgtaccc agtctggctc agcaaggagg ctgtcagcat cttgaaagct
     1921 ttcatgacga agaatcccca caagcgcctg ggctgtgtgg catcgcagaa tggcgaggac
     1981 gccatcaagc agcacccatt cttcaaagag attgactggg tgctcctgga gcagaagaag
     2041 atcaagccac ccttcaaacc acgcattaaa accaaaagag acgtcaataa ttttgaccaa
     2101 gactttaccc gggaagagcc ggtactcacc cttgtggacg aagcaattgt aaagcagatc
     2161 aaccaggagg aattcaaagg tttctcctac tttggtgaag acctgatgcc c
//
