LOCUS       sequence-155652-        5352 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing TSC2, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TSC2_WT
FEATURES             Location/Qualifiers
     source          1..5352
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atggccaaac caacaagcaa agattcaggc ttgaaggaga agtttaagat tctgttggga
       61 ctgggaacac cgaggccaaa tcccaggtct gcagagggta aacagacgga gtttatcatc
      121 accgcggaaa tactgagaga actgagcatg gaatgtggcc tcaacaatcg catccggatg
      181 atagggcaga tttgtgaagt cgcaaaaacc aagaaatttg aagagcacgc agtggaagca
      241 ctctggaagg cggtcgcgga tctgttgcag ccggagcggc cgctggaggc ccggcacgcg
      301 gtgctggctc tgctgaaggc catcgtgcag gggcagggcg agcgtttggg ggtcctcaga
      361 gccctcttct ttaaggtcat caaggattac ccttccaacg aagaccttca cgaaaggctg
      421 gaggttttca aggccctcac agacaatggg agacacatca cctacttgga ggaagagctg
      481 gctgactttg tcctgcagtg gatggatgtt ggcttgtcct cggaattcct tctggtgctg
      541 gtgaacttgg tcaaattcaa tagctgttac ctcgacgagt acatcgcaag gatggttcag
      601 atgatctgtc tgctgtgcgt ccggaccgtg tcctctgtgg acatagaggt ctccctgcag
      661 gtgctggacg ccgtggtctg ctacaactgc ctgccggctg agagcctccc gctgttcatc
      721 gttaccctct gtcgcaccat caacgtcaag gagctctgcg agccttgctg gaagctgatg
      781 cggaacctcc ttggcaccca cctgggccac agcgccatct acaacatgtg ccacctcatg
      841 gaggacagag cctacatgga ggacgcgccc ctgctgagag gagccgtgtt ttttgtgggc
      901 atggctctct ggggagccca ccggctctat tctctcagga actcgccgac atctgtgttg
      961 ccatcatttt accaggccat ggcatgtccg aacgaggtgg tgtcctatga gatcgtcctg
     1021 tccatcacca ggctcatcaa gaagtatagg aaggagctcc aggtggtggc gtgggacatt
     1081 ctgctgaaca tcatcgaacg gctccttcag cagctccaga ccttggacag cccggagctc
     1141 aggaccatcg tccatgacct gttgaccacg gtggaggagc tgtgtgacca gaacgagttc
     1201 cacgggtctc aggagagata ctttgaactg gtggagagat gtgcggacca gaggcctgag
     1261 tcctccctcc tgaacctgat ctcctataga gcgcagtcca tccacccggc caaggacggc
     1321 tggattcaga acctgcaggc gctgatggag agattcttca ggagcgagtc ccgaggcgcc
     1381 gtgcgcatca aggtgctgga cgtgctgtcc tttgtgctgc tcatcaacag gcagttctat
     1441 gaggaggagc tgattaactc agtggtcatc tcgcagctct cccacatccc cgaggataaa
     1501 gaccaccagg tccgaaagct ggccacccag ttgctggtgg acctggcaga gggctgccac
     1561 acacaccact tcaacagcct gctggacatc atcgagaagg tgatggcccg ctccctctcc
     1621 ccacccccgg agctggaaga aagggatgtg gccgcatact cggcctcctt ggaggatgtg
     1681 aagacagccg tcctggggct tctggtcatc cttcagacca agctgtacac cctgcctgca
     1741 agccacgcca cgcgtgtgta tgagatgctg gtcagccaca ttcagctcca ctacaagcac
     1801 agctacaccc tgccaatcgc gagcagcatc cggctgcagg cctttgactt cctgttgctg
     1861 ctgcgggccg actcactgca ccgcctgggc ctgcccaaca aggatggagt cgtgcggttc
     1921 agcccctact gcgtctgcga ctacatggag ccagagagag gctctgagaa gaagaccagc
     1981 ggcccccttt ctcctcccac agggcctcct ggcccggcgc ctgcaggccc cgccgtgcgg
     2041 ctggggtccg tgccctactc cctgctcttc cgcgtcctgc tgcagtgctt gaagcaggag
     2101 tctgactggg aggtgctgaa gctggttctg ggcaggctgc ctgagtccct gcgctataaa
     2161 gtgctcatct ttacttcccc ttgcagtgtg gaccagctgt gctctgctct ctgctccatg
     2221 ctttcaggcc caaagacact ggagcggctc cgaggcgccc cagaaggctt ctccagaact
     2281 gacttgcacc tggccgtggt tccagtgctg acagcattaa tctcttacca taactacctg
     2341 gacaaaacca aacagcgcga gatggtctac tgcctggagc agggcctcat ccaccgctgt
     2401 gccagccagt gcgtcgtggc cttgtccatc tgcagcgtgg agatgcctga catcatcatc
     2461 aaggcgctgc ctgttctggt ggtgaagctc acgcacatct cagccacagc cagcatggcc
     2521 gtcccactgc tggagttcct gtccactctg gccaggctgc cgcacctcta caggaacttt
     2581 gccgcggagc agtatgccag tgtgttcgcc atctccctgc cgtacaccaa cccctccaag
     2641 tttaatcagt acatcgtgtg tctggcccat cacgtcatag ccatgtggtt catcaggtgc
     2701 cgcctgccct tccggaagga ttttgtccct ttcatcacta agggcctgcg gtccaatgtc
     2761 ctcttgtctt ttgatgacac ccccgagaag gacagcttca gggcccggag tactagtctc
     2821 aacgagagac ccaagagtct gaggatagcc agacccccca aacaaggctt gaataactct
     2881 ccacccgtga aagaattcaa ggagagctct gcagccgagg ccttccggtg ccgcagcatc
     2941 agtgtgtctg aacatgtggt ccgcagcagg atacagacgt ccctcaccag tgccagcttg
     3001 gggtctgcag atgagaactc cgtggcccag gctgacgata gcatgaaaaa cctccacctg
     3061 gagctcacgg aaacctgtct ggacatgatg gctcgatacg tcttctccaa cttcacggct
     3121 gtcccgaaga ggtctcctgt gggcgagttc ctcctagcgg gtggcaggac caaaacctgg
     3181 ctggttggga acaagcttgt cactgtgacg acaagcgtgg gaaccgggac ccggtcgtta
     3241 ctaggcctgg actcggggga gctgcagtcc ggcccggagt cgagctccag ccccggggtg
     3301 catgtgagac agaccaagga ggcgccggcc aagctggagt cccaggctgg gcagcaggtg
     3361 tcccgtgggg cccgggatcg ggtccgttcc atgtcggggg gccatggtct tcgagttggc
     3421 gccctggacg tgccggcctc ccagttcctg ggcagtgcca cttctccagg accacggact
     3481 gcaccagccg cgaaacctga gaaggcctca gctggcaccc gggttcctgt gcaggagaag
     3541 acgaacctgg cggcctatgt gcccctgctg acccagggct gggcggagat cctggtccgg
     3601 aggcccacag ggaacaccag ctggctgatg agcctggaga acccgctcag ccctttctcc
     3661 tcggacatca acaacatgcc cctgcaggag ctgtctaacg ccctcatggc ggctgagcgc
     3721 ttcaaggagc accgggacac agccctgtac aagtcactgt cggtgccggc agccagcacg
     3781 gccaaacccc ctcctctgcc tcgctccaac acagactccg ccgtggtcat ggaggaggga
     3841 agtccgggcg aggttcctgt gctggtggag cccccagggt tggaggacgt tgaggcagcg
     3901 ctaggcatgg acaggcgcac ggatgcctac agcaggtcgt cctcagtctc cagccaggag
     3961 gagaagtcgc tccacgcgga ggagctggtt ggcaggggca tccccatcga gcgagtcgtc
     4021 tcctcggagg gtggccggcc ctctgtggac ctctccttcc agccctcgca gcccctgagc
     4081 aagtccagct cctctcccga gctgcagact ctgcaggaca tcctcgggga ccctggggac
     4141 aaggccgacg tgggccggct gagccctgag gttaaggccc ggtcacagtc agggaccctg
     4201 gacggggaaa gtgctgcctg gtcggcctcg ggcgaagaca gtcggggcca gcccgagggt
     4261 cccttgcctt ccagctcccc ccgctcgccc agtggcctcc ggccccgagg ttacaccatc
     4321 tccgactcgg ccccatcacg caggggcaag agagtagaga gggacgcctt aaagagcaga
     4381 gccacagcct ccaatgcaga gaaagtgcca ggcatcaacc ccagtttcgt gttcctgcag
     4441 ctctaccatt cccccttctt tggcgacgag tcaaacaagc caatcctgct gcccaatgag
     4501 tcacagtcct ttgagcggtc ggtgcagctc ctcgaccaga tcccatcata cgacacccac
     4561 aagatcgccg tcctgtatgt tggagaaggc cagagcaaca gcgagctcgc catcctgtcc
     4621 aatgagcatg gctcctacag gtacacggag ttcctgacgg gcctgggccg gctcatcgag
     4681 ctgaaggact gccagccgga caaggtgtac ctgggaggcc tggacgtgtg tggtgaggac
     4741 ggccagttca cctactgctg gcacgatgac atcatgcaag ccgtcttcca catcgccacc
     4801 ctgatgccca ccaaggacgt ggacaagcac cgctgcgaca agaagcgcca cctgggcaac
     4861 gactttgtgt ccattgtcta caatgactcc ggtgaggact tcaagcttgg caccatcaag
     4921 ggccagttca actttgtcca cgtgatggtc accccgctgg actacgagtg caacctggtg
     4981 tccctgcagt gcaggaaaga catggagggc cttgtggaca ccagcgtggc caagatcgtg
     5041 tctgaccgca acctgccctt cgtggcccgc cagatggccc tgcacgcaaa tatggcctca
     5101 caggtgcatc atagccgctc caaccccacc gatatctacc cctccaagtg gattgcccgg
     5161 ctccgccaca tcaagcggct ccgccagcgg atctgcgagg aagccgccta ctccaacccc
     5221 agcctacctc tggtgcaccc tccgtcccat agcaaagccc ctgcacagac tccagccgag
     5281 cccacacctg gctatgaggt gggccagcgg aagcgcctca tctcctcggt ggaggacttc
     5341 accgagtttg tg
//
