LOCUS       sequence-155663-        3705 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway Donor vector containing NOTCH2, part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_NOTCH2_WT
FEATURES             Location/Qualifiers
     source          1..3705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1736..1745
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgcccgccc tgcgccccgc tctgctgtgg gcgctgctgg cgctctggct gtgctgcgcg
       61 gcccccgcgc atgcattgca gtgtcgagat ggctatgaac cctgtgtaaa tgaaggaatg
      121 tgtgttacct accacaatgg cacaggatac tgcaaatgtc cagaaggctt cttgggggaa
      181 tattgtcaac atcgagaccc ctgtgagaag aaccgctgcc agaatggtgg gacttgtgtg
      241 gcccaggcca tgctggggaa agccacgtgc cgatgtgcct cagggtttac aggagaggac
      301 tgccagtact caacatctca tccatgcttt gtgtctcgac cctgcctgaa tggcggcaca
      361 tgccatatgc tcagccggga tacctatgag tgcacctgtc aagtcgggtt tacaggtaag
      421 gagtgccaat ggacggatgc ctgcctgtct catccctgtg caaatggaag tacctgtacc
      481 actgtggcca accagttctc ctgcaaatgc ctcacaggct tcacagggca gaaatgtgag
      541 actgatgtca atgagtgtga cattccagga cactgccagc atggtggcac ctgcctcaac
      601 ctgcctggtt cctaccagtg ccagtgccct cagggcttca caggccagta ctgtgacagc
      661 ctgtatgtgc cctgtgcacc ctcaccttgt gtcaatggag gcacctgtcg gcagactggt
      721 gacttcactt ttgagtgcaa ctgccttcca ggttttgaag ggagcacctg tgagaggaat
      781 attgatgact gccctaacca caggtgtcag aatggagggg tttgtgtgga tggggtcaac
      841 acttacaact gccgctgtcc cccacaatgg acaggacagt tctgcacaga ggatgtggat
      901 gaatgcctgc tgcagcccaa tgcctgtcaa aatgggggca cctgtgccaa ccgcaatgga
      961 ggctatggct gtgtatgtgt caacggctgg agtggagatg actgcagtga gaacattgat
     1021 gattgtgcct tcgcctcctg tactccaggc tccacctgca tcgaccgtgt ggcctccttc
     1081 tcttgcatgt gcccagaggg gaaggcaggt ctcctgtgtc atctggatga tgcatgcatc
     1141 agcaatcctt gccacaaggg ggcactgtgt gacaccaacc ccctaaatgg gcaatatatt
     1201 tgcacctgcc cacaaggcta caaaggggct gactgcacag aagatgtgga tgaatgtgcc
     1261 atggccaata gcaatccttg tgagcatgca ggaaaatgtg tgaacacgga tggcgccttc
     1321 cactgtgagt gtctgaaggg ttatgcagga cctcgttgtg agatggacat caatgagtgc
     1381 cattcagacc cctgccagaa tgatgctacc tgtctggata agattggagg cttcacatgt
     1441 ctgtgcatgc caggtttcaa aggtgtgcat tgtgaattag aaataaatga atgtcagagc
     1501 aacccttgtg tgaacaatgg gcagtgtgtg gataaagtca atcgtttcca gtgcctgtgt
     1561 cctcctggtt tcactgggcc agtttgccag attgatattg atgactgttc cagtactccg
     1621 tgtctgaatg gggcaaagtg tatcgatcac ccgaatggct atgaatgcca gtgtgccaca
     1681 ggtttcactg gtgtgttgtg tgaggagaac attgacaact gtgaccccga tccttgccac
     1741 catggtcagt gtcaggatgg tattgattcc tacacctgca tctgcaatcc cgggtacatg
     1801 ggcgccatct gcagtgacca gattgatgaa tgttacagca gcccttgcct gaacgatggt
     1861 cgctgcattg acctggtcaa tggctaccag tgcaactgcc agccaggcac gtcaggggtt
     1921 aattgtgaaa ttaattttga tgactgtgca agtaaccctt gtatccatgg aatctgtatg
     1981 gatggcatta atcgctacag ttgtgtctgc tcaccaggat tcacagggca gagatgtaac
     2041 attgacattg atgagtgtgc ctccaatccc tgtcgcaagg gtgcaacatg tatcaacggt
     2101 gtgaatggtt tccgctgtat atgccccgag ggaccccatc accccagctg ctactcacag
     2161 gtgaacgaat gcctgagcaa tccctgcatc catggaaact gtactggagg tctcagtgga
     2221 tataagtgtc tctgtgatgc aggctgggtt ggcatcaact gtgaagtgga caaaaatgaa
     2281 tgcctttcga atccgtgcca gaatggagga acttgtgaca atctggtgaa tggatacagg
     2341 tgtacttgca agaagggctt taaaggctat aactgccagg tgaatattga tgaatgtgcc
     2401 tcaaatccat gcctgaacca aggaacctgc tttgatgaca taagtggcta cacttgccac
     2461 tgtgtgctgc catacacagg caagaattgt cagacagtat tggctccctg ttccccaaac
     2521 ccttgtgaga atgctgctgt ttgcaaagag tcaccaaatt ttgagagtta tacttgcttg
     2581 tgtgctcctg gctggcaagg tcagcggtgt accattgaca ttgacgagtg tatctccaag
     2641 ccctgcatga accatggtct ctgccataac acccagggca gctacatgtg tgaatgtcca
     2701 ccaggcttca gtggtatgga ctgtgaggag gacattgatg actgccttgc caatccttgc
     2761 cagaatggag gttcctgtat ggatggagtg aatactttct cctgcctctg ccttccgggt
     2821 ttcactgggg ataagtacca gacagacatg aatgagtgtc tgagtgaacc ctgtaagaat
     2881 ggagggacct gctctgacta cgtcaacagt tacacttgca agtgccaggc aggatttgat
     2941 ggagtccatt gtgagaacaa catcaatgag tgcactgaga gctcctgttt caatggtggc
     3001 acatgtgttg atgggattaa ctccttctct tgcttgtgcc ctgtgggttt cactggatcc
     3061 ttctgcctcc atgagatcaa tgaatgcagc tctcatccat gcctgaatga gggaacgtgt
     3121 gttgatggcc tgggtaccta ccgctgcagc tgccccctgg gctacactgg gaaaaactgt
     3181 cagaccctgg tgaatctctg cagtcggtct ccatgtaaaa acaaaggtac ttgcgttcag
     3241 aaaaaagcag agtcccagtg cctatgtcca tctggatggg ctggtgccta ttgtgacgtg
     3301 cccaatgtct cttgtgacat agcagcctcc aggagaggtg tgcttgttga acacttgtgc
     3361 cagcactcag gtgtctgcat caatgctggc aacacgcatt actgtcagtg ccccctgggc
     3421 tatactggga gctactgtga ggagcaactc gatgagtgtg cgtccaaccc ctgccagcac
     3481 ggggcaacat gcagtgactt cattggtgga tacagatgcg agtgtgtccc aggctatcag
     3541 ggtgtcaact gtgagtatga agtggatgag tgccagaatc agccctgcca gaatggaggc
     3601 acctgtattg accttgtgaa ccatttcaag tgctcttgcc caccaggcac tcggggtatg
     3661 aaatcatcct tatccatttt ccatccaggg cattgtctta agtta
//
