LOCUS       sequence-155704-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 485, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: sfGFP-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      228..237
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(234..236,237..239,240..752)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVE"
     primer_bind     complement(279..300)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat ctggtacccc
       61 ttgcagcttc cagttgtcag gaagagaagg ctcccatgga tcctgtggtg gtgctggggc
      121 tctgtctctc ctgtttgctt ctcctttcac tctggaaaca gagctatggg ggagggaagc
      181 ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga
      241 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      301 taaacggcca caagttcagc gtgcgcggcg agggcgaggg cgatgccacc aacggcaagc
      361 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      421 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcgccacg
      481 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc agcttcaagg
      541 acgacggcac ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      601 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      661 agtacaactt caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca
      721 aggccaactt caagatccgc cacaacgtgg aggacggcag cgtgcagctc gccgaccact
      781 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
      841 gcacccagtc cgtgctgag
//
