LOCUS       sequence-155705-         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Ordon J, Gantner J, Kemna J, Schwalgun L, Reschke M, Streubel J, 
            Boch J, Stuttmann J
  TITLE     Generation of chromosomal deletions in dicotyledonous plants 
            employing a user-friendly genome editing toolkit.
  JOURNAL   Plant J. 2016 Aug 31. doi: 10.1111/tpj.13319.
  PUBMED    27579989
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2016 Aug 31. doi: 10.1111/tpj.13319."
COMMENT     Sequence Label: pDGE76
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(50..152)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        592..937
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     880..900
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
       61 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
      121 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ggtctctgca agaattcaag
      181 cttggaggtc aacatggtgg agcacgacac tctggtctac tccaaaaatg tcaaagatac
      241 agtctcagaa gatcaaaggg ctattgagac ttttcaacaa aggataattt cgggaaacct
      301 cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag aaaaggaagg
      361 tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag atctctctgc
      421 cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagaggt
      481 tccaaccacg tctacaaagc aagtggattg atgtgataac atggtggagc acgacactct
      541 ggtctactcc aaaaatgtca aagatacagt ctcagaagat caaagggcta ttgagacttt
      601 tcaacaaagg ataatttcgg gaaacctcct cggattccat tgcccagcta tctgtcactt
      661 catcgaaagg acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg
      721 aaaggctatc attcaagatc tctctgccga cagtggtccc aaagatggac ccccacccac
      781 gaggagcatc gtggaaaaag aagaggttcc aaccacgtct acaaagcaag tggattgatg
      841 tgacatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc
      901 ctctatataa ggaagttcat ttcatttgga gaggacacgc tcgagtataa gagctcattt
      961 t
//
