LOCUS       sequence-155708-         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pX335 vector encoding SpCas9n and a chimeric guide RNA targeting 
            Prnp coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS
  TITLE     Manipulating the Prion Protein Gene Sequence and Expression Levels 
            with CRISPR/Cas9.
  JOURNAL   PLoS One. 2016 Apr 29;11(4):e0154604. doi: 
            10.1371/journal.pone.0154604. eCollection 2016.
  PUBMED    27128441
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-04-29"; volume: "11(4):e0154604. doi"; pages: " 
            10.1371/journal.pone.0154604. eCollection 2016"
COMMENT     Sequence Label: pX335_HR_Prnp_3
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..42)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taggaattct tagctggcct ccacctttct cttcttcttg gggctgtcgc ctcccagctg
       61 agacaggtcg atccgtgtct cgtacaggcc ggtgatgctc tggtggatca gggtggcgtc
      121 cagcacctct ttggtgctgg tgtacctctt ccggtcgatg gtggtgtcaa agtacttgaa
      181 ggcggcaggg gctcccagat tggtcagggt aaacaggtgg atgatattct cggcctgctc
      241 tctgatgggc ttatcccggt gcttgttgta ggcggacagc actttgtcca gattagcgtc
      301 ggccaggatc actctcttgg agaactcgct gatctgctcg atgatctcgt ccaggtagtg
      361 cttgtgctgt tccacaaaca gctgtttctg ctcattatcc tcgggggagc ccttcagctt
      421 ctcatagtgg ctggccaggt acaggaagtt cacatatttg gagggcaggg ccagttcgtt
      481 tcccttctgc agttcgccgg cagaggccag cattctcttc cggccgtttt ccagctcgaa
      541 cagggagtac ttaggcagct tgatgatcag gtcctttttc acttctttgt agcccttggc
      601 ttccagaaag tcgatgggat tcttctcgaa gctgcttctt tccatgatgg tgatccccag
      661 cagctctttc acactcttca gtttcttgga cttgcccttt tccactttgg ccaccaccag
      721 cacagaatag gccacggtgg ggctgtcgaa gccgccgtac ttcttagggt cccagtcctt
      781 ctttctggcg atcagcttat cgctgttcct cttgggcagg atagactctt tgctgaagcc
      841 gcctgtctgc acctcggtct ttttcacgat attcacttgg ggcatgctca gcactttccg
      901 cacggtggca aaatcccggc ccttatccca cacgatctcc ccggtttcg
//
